ZFIN ID: ZDB-PUB-090112-17
Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation
Bukrinsky, A., Griffin, K.J., Zhao, Y., Lin, S., and Banerjee, U.
Date: 2009
Source: Blood   113(9): 2038-2046 (Journal)
Registered Authors: Bukrinsky, Alex, Griffin, Kevin, Lin, Shuo, Zhao, Yan
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/genetics*
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental/drug effects
  • Molecular Sequence Data
  • Morpholines/chemistry
  • Morpholines/pharmacology
  • Myeloid Cells/metabolism
  • Myeloid Cells/physiology*
  • Oligonucleotides, Antisense/pharmacology
  • Phylogeny
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins/chemistry
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology
  • Sequence Homology, Amino Acid
  • Trans-Activators/chemistry
  • Trans-Activators/genetics
  • Trans-Activators/physiology
  • Transcription Factors/chemistry
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription Factors/physiology*
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed: 19131555 Full text @ Blood
The ETS protein Spi-1/Pu.1 plays a pivotal and widespread role throughout hematopoiesis in many species. This study describes the identification, characterization and functional analysis of a new zebrafish spi transcription factor spi-1-like (spi-1l) that is expressed in primitive myeloid cells, erythro-myelo progenitor cells, and in the adult kidney. Spi-1l functions genetically downstream of etsrp, scl, and spi-1/pu.1 in myeloid differentiation. Spi-1l is co-expressed in a subset of spi-1/pu.1 cells and its function is necessary and sufficient for macrophage and granulocyte differentiation. These results establish a critical role for spi-1l in zebrafish myeloid cell differentiation.