ZFIN ID: ZDB-PUB-090106-2
Muscle degeneration and leukocyte infiltration caused by mutation of zebrafish fad24
Walters, K.B., Dodd, M.E., Mathias, J.R., Gallagher, A.J., Bennin, D.A., Rhodes, J., Kanki, J.P., Look, A.T., Grinblat, Y., and Huttenlocher, A.
Date: 2009
Source: Developmental dynamics : an official publication of the American Association of Anatomists   238(1): 86-99 (Journal)
Registered Authors: Dodd, M. Ernest, Gallagher, Andrea, Grinblat, Yevgenya, Huttenlocher, Anna, Kanki, John, Look, A. Thomas, Mathias, Jonathan, Rhodes, Jennifer, Walters, Kevin
Keywords: zebrafish, fad24, neutrophil, inflammation
MeSH Terms:
  • Animals
  • Apoptosis/physiology
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Basic-Leucine Zipper Transcription Factors/genetics
  • Basic-Leucine Zipper Transcription Factors/metabolism*
  • Cell Nucleus/metabolism
  • Humans
  • In Situ Hybridization
  • Leukocytes/metabolism*
  • Lipid Metabolism
  • Muscle, Skeletal/cytology
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/pathology
  • Muscular Atrophy*/genetics
  • Muscular Atrophy*/pathology
  • Mutation*
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism*
  • Oligonucleotides, Antisense/metabolism
  • Zebrafish*/anatomy & histology
  • Zebrafish*/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 19097072 Full text @ Dev. Dyn.
Factor for adipocyte differentiation 24 (fad24) is a novel gene that has been implicated in adipocyte differentiation and DNA replication. In a screen for zebrafish mutants that have an abnormal tissue distribution of neutrophils, we identified an insertional allele of fad24, fad24(hi1019). Homozygous fad24(hi1019) larvae exhibit muscle degeneration accompanied by leukocyte infiltration. Muscle degeneration was extensive and included tissue apoptosis and disorganized, poorly striated muscle fibers. Blocking apoptosis using pan-caspase inhibitors resulted in decreased neutrophil recruitment into the body of the larva, suggesting a causative link between apoptosis and leukocyte infiltration. These findings suggest that zebrafish is a powerful genetic model system to address the interplay between muscle degeneration and leukocyte infiltration, and indicate that tissue apoptosis may contribute to neutrophil recruitment in some inflammatory states. Developmental Dynamics 238:86-99, 2009. (c) 2008 Wiley-Liss, Inc.