ZFIN ID: ZDB-PUB-090106-1
The extracellular matrix protein TGFBI promotes myofibril bundling and muscle fibre growth in the zebrafish embryo
Kim, H.R., and Ingham, P.W.
Date: 2009
Source: Developmental dynamics : an official publication of the American Association of Anatomists   238(1): 56-65 (Journal)
Registered Authors: Ingham, Philip, Kim, Rosemary
Keywords: Tgfbi, extracellular matrix, skeletal muscle, myofibril, zebrafish, embryo
MeSH Terms:
  • Animals
  • Extracellular Matrix/metabolism*
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/metabolism*
  • Humans
  • Mice
  • Muscle Fibers, Skeletal/cytology
  • Muscle Fibers, Skeletal/physiology*
  • Myofibrils/physiology*
  • Myofibrils/ultrastructure
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism*
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 19097068 Full text @ Dev. Dyn.
The extracellular matrix protein Tgfbi has been shown to localise to myotendinous junctions in mouse and postulated to interact with the transmembrane protein Integrin alpha7beta1, which, in parallel with the Dystrophin-associated protein complex, is critical for linkage between the extracellular matrix and the cytoskeleton of muscle fibres. Here we use a GFP-tagged form of Tgfbi to analyse its distribution in the developing skeletal muscle of the zebrafish embryos and antisense morpholino oligonucleotides to investigate the function of the endogenous protein. We find that although tagged Tgfbi accumulates at the myosepta, the attachment of muscle fibres to the myosepta is established and maintained normally in morphant embryos; however, the fibres show a marked reduction in their growth and a disruption of their myofibril bundles.