PUBLICATION

Enantioselective induction of estrogen-responsive gene expression by permethrin enantiomers in embryo-larval zebrafish

Authors
Jin, Y., Chen, R., Sun, L., Wang, W., Zhou, L., Liu, W., and Fu, Z.
ID
ZDB-PUB-090105-12
Date
2009
Source
Chemosphere   74(9): 1238-1244 (Journal)
Registered Authors
Keywords
Zebrafish, Permethrin enantiomers, Gene expression, Enantioselectivity
MeSH Terms
  • Animals
  • Aromatase/metabolism
  • Chromatography, High Pressure Liquid
  • DNA Primers/genetics
  • Dose-Response Relationship, Drug
  • Estrogens/metabolism*
  • Gene Expression Regulation/drug effects*
  • Insecticides/chemistry
  • Insecticides/toxicity*
  • Permethrin/chemistry
  • Permethrin/toxicity*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stereoisomerism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism
PubMed
19095286 Full text @ Chemosphere
Abstract
Enantioselectivity in the separation, toxicology, biodegradation and estrogenic activity of chiral pesticides has become a groundbreaking topic recently. In this study, real-time, quantitative polymerase chain reaction was adapted to investigate the induction of estrogen-responsive gene expression in embryo-larval zebrafish after 7d of exposure to permethrin (PM) enantiomers. The PM enantiomers were completely separated by a chiral HPLC column. The in vivo study found that a 7d exposure to 250ngL(-1) PM racemate and its enantiomers was sufficient to stimulate vtg1, esralpha and cyp19b expression, while 1000ngL(-1) exposure significantly induced gene expression in a pattern similar to that of the control (50ngL(-1) E2), except for vtg2. Significant differences were detected between the enantiomers in the induction of estrogen-responsive gene expression. At the exposure level of 1000ngL(-1), the vtg1, esralpha and cyp19b responses to the (-)-trans enantiomer were about 3.2-, 1.8- and 1.5-fold higher, respectively, than those in the group treated with (+)-trans enantiomer (p<0.05). In the two cis-enantiomer treatment groups, (+)-cis increased the mRNA level of the cyp19b gene about 1.5-fold higher than the (-)-cis-enantiomer did. Of the four enantiomers, the (-)-trans enantiomer showed the greatest estrogenic activity. The results strongly indicate the occurrence of significant enantioselectivity in estrogenic activity of PM enantiomers exposed to embryo-larval zebrafish. These findings add to a growing body of evidence concerning enantioselectivity in the toxicity, endocrine-disrupting activity, and environmental biodegradation of chiral pesticides.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes