PUBLICATION

Chapter 4. Using the zebrafish to study vessel formation

Authors
McKinney, M.C., and Weinstein, B.M.
ID
ZDB-PUB-081121-1
Date
2008
Source
Methods in enzymology   444: 65-97 (Chapter)
Registered Authors
Weinstein, Brant M.
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian/transplantation
  • Models, Animal
  • Neovascularization, Physiologic*/genetics
  • Zebrafish*/embryology
  • Zebrafish*/genetics
PubMed
19007661 Full text @ Methods Enzymol.
Abstract
Danio rerio, commonly referred to as the zebrafish, is a powerful animal model for studying the formation of the vasculature. Zebrafish offer unique opportunities for in vivo analysis of blood and lymphatic vessels formation because of their accessibility to large-scale genetic and experimental analysis as well as the small size, optical clarity, and external development of zebrafish embryos and larvae. A wide variety of established techniques are available to study vessel formation in the zebrafish, from early endothelial cell differentiation to adult vessel patterning. In this chapter, we review methods used to functionally manipulate and visualize the vasculature in the zebrafish and illustrate how these methods have helped further understanding of the genetic components regulating formation and patterning of developing vessels.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes