PUBLICATION

Antiangiogenic activity of Tripterygium wilfordii and its terpenoids

Authors
He, M.F., Liu, L., Ge, W., Shaw, P.C., Jiang, R., Wu, L.W., and But, P.P.
ID
ZDB-PUB-081114-5
Date
2009
Source
Journal of ethnopharmacology   121(1): 61-68 (Journal)
Registered Authors
Ge, Wei
Keywords
Antiangiogenesis, Zebrafish, Tripterygium wilfordii, Triptolide, Angpt2, Tie2
MeSH Terms
  • Angiogenesis Inhibitors/chemistry
  • Angiogenesis Inhibitors/pharmacology*
  • Animals
  • Disease Models, Animal
  • Diterpenes/chemistry
  • Diterpenes/pharmacology
  • Drugs, Chinese Herbal/pharmacology
  • Embryo, Nonmammalian/blood supply
  • Epoxy Compounds/chemistry
  • Epoxy Compounds/pharmacology
  • Neovascularization, Physiologic/drug effects
  • Phenanthrenes/chemistry
  • Phenanthrenes/pharmacology
  • Plant Roots/chemistry
  • Reverse Transcriptase Polymerase Chain Reaction
  • Terpenes/chemistry
  • Terpenes/pharmacology*
  • Tripterygium/chemistry*
  • Triterpenes/chemistry
  • Triterpenes/pharmacology
  • Zebrafish
PubMed
18996177 Full text @ J. Ethnopharmacol.
Abstract
Tripterygium wilfordii Hook. f. (Celastraceae) has been traditionally used as folk medicine for centuries in China for the treatment of immune-inflammatory diseases. This study aimed to assess the antiangiogenic activities which support the therapeutic use of Tripterygium wilfordii and its terpenoids for angiogenesis disease such as cancer. The ethanol extract of Tripterygium wilfordii and subsequent fractions were evaluated on an in vivo antiangiogenic zebrafish embryo model. Three antiangiogenic terpenoids were isolated by bioassay-guided purification, namely, celastrol (4), cangoronine (5) and triptolide (7). Among them, triptolide manifested the most potent antiangiogenic activity against vessel formation by nearly 50% at 1.2muM. Semi-quantitative RT-PCR analysis revealed that triptolide dose- and time-dependently reduced the mRNA expression of angiopoietin (angpt)2 and tie2 in zebrafish, indicating the involvement of angpt2/tie2 signaling pathway in the antiangiogenic action of triptolide. The discovery of an alternative pathway further confirms the value of ethnopharmacological investigations into traditional botanicals for leads for potential drug development.
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