PUBLICATION
Protective effects of vitamin E against 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) induced toxicity in zebrafish embryos
- Authors
- Na, Y.R., Seok, S.H., Baek, M.W., Lee, H.Y., Kim, D.J., Park, S.H., Lee, H.K., and Park, J.H.
- ID
- ZDB-PUB-081105-3
- Date
- 2009
- Source
- Ecotoxicology and environmental safety 72(3): 714-719 (Journal)
- Registered Authors
- Keywords
- 3, 3′, 4, 4′, 5-Pentachlorobiphenyl (PCB126), Vitamin E, Zebrafish (Danio rerio) embryo, CYP1A, HSC70, SOD2
- MeSH Terms
-
- Animals
- Antioxidants/pharmacology*
- Drug Antagonism
- Edema/chemically induced
- Edema/pathology
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/physiopathology
- Embryonic Development/drug effects
- Estrogen Antagonists/toxicity*
- Gene Expression/drug effects
- Oxidative Stress/drug effects
- Pericardium/drug effects
- Pericardium/pathology
- Polychlorinated Biphenyls/toxicity*
- RNA, Messenger/metabolism
- Vitamin E/pharmacology*
- Water Pollutants, Chemical/toxicity*
- Yolk Sac/drug effects
- Yolk Sac/pathology
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 18973944 Full text @ Ecotoxicol. Environ. Saf.
Citation
Na, Y.R., Seok, S.H., Baek, M.W., Lee, H.Y., Kim, D.J., Park, S.H., Lee, H.K., and Park, J.H. (2009) Protective effects of vitamin E against 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) induced toxicity in zebrafish embryos. Ecotoxicology and environmental safety. 72(3):714-719.
Abstract
3,3',4,4',5-Pentachlorinated biphenyls 126 (PCB126) is a global environmental contaminant that can induce cellular oxidative stress. We investigated whether vitamin E can protect against toxicity from PCB126 during zebrafish (Danio rerio) development. Zebrafish embryos were exposed to 100nM PCB126 and compared with a second group that was co-exposed with 100muM vitamin E until 5 days post fertilization. PCB126 induced pericardial sac edema, yolk sac edema, and growth retardation in zebrafish embyos. In contrast, vitamin E co-exposure group did not show any gross changes. Real-time PCR results showed that vitamin E co-exposure group were restored to control group for the expression levels of heat shock protein 70 Cognate, aryl hydrocarbon receptor type-2, cytochrome P450 1A, and superoxide dismutase-1. These data give insights into the use of vitamin E to reduce PCB126-mediated toxicity and into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of AHR agonists.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping