PUBLICATION
Involvement of estradiol-17beta and its membrane receptor, G protein coupled receptor 30 (GPR30) in regulation of oocyte maturation in zebrafish, Danio rario
- Authors
- Pang, Y., and Thomas, P.
- ID
- ZDB-PUB-081029-21
- Date
- 2009
- Source
- General and comparative endocrinology 161(1): 58-61 (Journal)
- Registered Authors
- Keywords
- G protein-coupled receptor 30, GPR30, Oocyte maturation, Aromatase inhibitor, Meiosis arrest
- MeSH Terms
-
- Zebrafish
- Tamoxifen/pharmacology
- Hydroxyprogesterones/pharmacology
- Quinolines/pharmacology
- Female
- Receptors, Estradiol/agonists
- Receptors, Estradiol/physiology*
- Zebrafish Proteins/physiology*
- Receptors, G-Protein-Coupled/agonists
- Receptors, G-Protein-Coupled/physiology*
- Animals
- Androstatrienes/pharmacology
- Estradiol/analogs & derivatives
- Estradiol/metabolism*
- Estradiol/pharmacology
- Aromatase Inhibitors/pharmacology
- Oocytes/physiology*
- Cyclopentanes/pharmacology
- PubMed
- 18952087 Full text @ Gen. Comp. Endocrinol.
Citation
Pang, Y., and Thomas, P. (2009) Involvement of estradiol-17beta and its membrane receptor, G protein coupled receptor 30 (GPR30) in regulation of oocyte maturation in zebrafish, Danio rario. General and comparative endocrinology. 161(1):58-61.
Abstract
The orphan G protein coupled receptor, GPR30, has the characteristics of a high affinity, specific estrogen membrane receptor on Atlantic croaker oocytes and mediates estrogen inhibition of oocyte maturation in this perciform fish. In order to determine the broad applicability of these findings to other teleosts, similar experiments were conducted in a cyprinid fish, zebrafish, in the present study. GPR30 mRNA expression was detected in zebrafish oocytes but not in the ovarian follicular cells. Both spontaneous and 17, 20beta-dihyroxy-4-pregnen-3-one (DHP)-induced maturation of follicle-enclosed zebrafish oocytes was significantly decreased when they were incubated with either estradiol-17beta, or the GPR30 agonists, ICI 182 780 and tamoxifen, or with the GPR30 specific agonist G-1. On the other hand spontaneous oocyte maturation increased two-fold when zebrafish ovarian follicles were incubated with an aromatase inhibitor, ATD. Moreover, the stimulatory effects of ATD on germinal vesicle breakdown (GVBD) were partially reversed by co-treatment with 100 nM of E2 or G-1. These results suggest that endogenous estrogens acting through GPR30 are involved in maintaining meiotic arrest of zebrafish oocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping