PUBLICATION

In vivo Analysis of Choroid Plexus Morphogenesis in Zebrafish

Authors

García-Lecea, M., Kondrychyn, I., Fong, S.H., Ye, Z.R., and Korzh, V.

ID

ZDB-PUB-080908-11

Date

2008

Source

PLoS One 3(9): e3090 (Journal)

Registered Authors

Fong, Steven, Kondrychyn, Igor, Korzh, Vladimir

Keywords

Embryos, Fourth ventricle, Zebrafish, Morphogenesis, Hindbrain, Apoptosis, Choroid plexus, Hedgehog signaling

MeSH Terms

Morphogenesis*
Choroid Plexus/physiology*
Animals, Genetically Modified
Models, Biological
Animals
Blood-Brain Barrier
Apoptosis
Microscopy/methods
Green Fluorescent Proteins/metabolism
Mutation
Cell Lineage
Receptors, Notch/metabolism
Zebrafish
Embryo, Nonmammalian/physiology
Signal Transduction

(all 15)

PubMed

18769618 Full text @ PLoS One

Abstract

BACKGROUND: The choroid plexus (ChP), a component of the blood-brain barrier (BBB), produces the cerebrospinal fluid (CSF) and as a result plays a role in (i) protecting and nurturing the brain as well as (ii) in coordinating neuronal migration during neurodevelopment. Until now ChP development was not analyzed in living vertebrates due to technical problems. METHODOLOGY/PRINCIPAL FINDINGS: We have analyzed the formation of the fourth ventricle ChP of zebrafish in the GFP-tagged enhancer trap transgenic line SqET33-E20 (Gateways) by a combination of in vivo imaging, histology and mutant analysis. This process includes the formation of the tela choroidea (TC), the recruitment of cells from rhombic lips and, finally, the coalescence of TC resulting in formation of ChP. In Notch-deficient mib mutants the first phase of this process is affected with premature GFP expression, deficient cell recruitment into TC and abnormal patterning of ChP. In Hedgehog-deficient smu mutants the second phase of the ChP morphogenesis lacks cell recruitment and TC cells undergo apoptosis. CONCLUSIONS/SIGNIFICANCE: This study is the first to demonstrate the formation of ChP in vivo revealing a role of Notch and Hedgehog signalling pathways during different developmental phases of this process.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
csrnp1b	sqet33e20Et/sqet33e20Et	standard conditions	Long-pec	hindbrain anterior-posterior axis	ISH	Fig. 1
csrnp1b	sqet33e20Et/sqet33e20Et	standard conditions	Long-pec	hindbrain dorsal side	ISH	Fig. 1
EGFP	mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Prim-5	fourth ventricle roof plate	IFL	text only
EGFP	mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	hindbrain	ISH	Fig. 6
EGFP	mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Prim-25	tela chorioidea	IFL	Fig. 5
EGFP	mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	tela chorioidea	IFL	Fig. 5
EGFP	mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Pec-fin	tela chorioidea	IFL	Fig. 5
EGFP	smo^b641/b641; sqet33e20Et	standard conditions	Long-pec	hindbrain	ISH	Fig. 6
EGFP	smo^b641/b641; sqet33e20Et	standard conditions	Long-pec	tela chorioidea	IFL	Fig. 5
EGFP	smo^b641/b641; sqet33e20Et	standard conditions	Pec-fin	tela chorioidea	IFL	Fig. 5

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	cell division process quality, normal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	rhombic lip cellular motility, abnormal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	tela chorioidea cell fragility, normal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	tela chorioidea cell normal amount, normal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	tela chorioidea mislocalised, abnormal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Long-pec	tela chorioidea morphology, abnormal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Pec-fin	rhombic lip cellular motility, abnormal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Pec-fin	tela chorioidea cell normal amount, normal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Pec-fin	tela chorioidea mislocalised, abnormal	Fig. 5
mib1^tfi91/tfi91; sqet33e20Et	standard conditions	Pec-fin	tela chorioidea morphology, abnormal	Fig. 5

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b641		Point Mutation	smo
sqet33e20Et	Et(krt4:EGFP)	Transgenic Insertion
sqgw42aEt	Et(krt4:EGFP)	Transgenic Insertion
sqgw42B1Et	Et(krt4:EGFP)	Transgenic Insertion
sqgw45cEt	Et(krt4:EGFP)	Transgenic Insertion
tfi91		Point Mutation	mib1

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
mib1^tfi91/tfi91; sqet33e20Et
smo^b641/b641; sqet33e20Et
sqet33e20Et/sqet33e20Et
sqgw42aEt
sqgw42B1Et
sqgw45cEt

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab5-GFP	monoclonal	GFP	IgG2a	Mouse

Orthology

Gene	Orthology
slco5a1a

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Entity Type	Entity Symbol	Location
Feature	sqet33e20Et	Chr: 24 Details
Feature	sqgw42aEt	Chr: 24 Details
Feature	sqgw42B1Et	Chr: 24 Details
Feature	sqgw45cEt	Chr: 24 Details
GENE	csrnp1b	Chr: 24 Details

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García-Lecea et al., 2008 ZDB-PUB-080908-11 PMID:18769618

In vivo Analysis of Choroid Plexus Morphogenesis in Zebrafish

García-Lecea et al., 2008

ZDB-PUB-080908-11
PMID:18769618