PUBLICATION

Development and notch signaling requirements of the zebrafish choroid plexus

Authors
Bill, B.R., Balciunas, D., McCarra, J.A., Young, E.D., Xiong, T., Spahn, A.M., Garcia-Lecea, M., Korzh, V., Ekker, S.C., and Schimmenti, L.A.
ID
ZDB-PUB-080908-10
Date
2008
Source
PLoS One   3(9): e3114 (Journal)
Registered Authors
Balciunas, Darius, Bill, Brent, Ekker, Stephen C., Garcia-Lecea, Marta, Korzh, Vladimir
Keywords
Zebrafish, Notch signaling, Larvae, Morpholino, Fourth ventricle, Choroid plexus, Cell migration, Fluorescence imaging
MeSH Terms
  • Animals
  • Biological Transport
  • Blood-Brain Barrier
  • Brain/metabolism
  • Choroid Plexus/embryology*
  • Choroid Plexus/metabolism*
  • Epithelium/metabolism
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins/metabolism
  • Microscopy, Fluorescence
  • Models, Biological
  • Receptors, Notch/metabolism*
  • Signal Transduction
  • Zebrafish
  • Zebrafish Proteins/metabolism
PubMed
18769591 Full text @ PLoS One
Abstract
BACKGROUND: The choroid plexus (CP) is an epithelial and vascular structure in the ventricular system of the brain that is a critical part of the blood-brain barrier. The CP has two primary functions, 1) to produce and regulate components of the cerebral spinal fluid, and 2) to inhibit entry into the brain of exogenous substances. Despite its importance in neurobiology, little is known about how this structure forms. METHODOLOGY AND PRINCIPAL FINDINGS: Here we show that the transposon-mediated enhancer trap zebrafish line Et(Mn16) expresses green fluorescent protein within a population of cells that migrate toward the midline and coalesce to form the definitive CP. We further demonstrate the development of the integral vascular network of the definitive CP. Utilizing pharmacologic pan-notch inhibition and specific morpholino-mediated knockdown, we demonstrate a requirement for Notch signaling in choroid plexus development. We identify three Notch signaling pathway members as mediating this effect, notch1b, deltaA, and deltaD. CONCLUSIONS AND SIGNIFICANCE: This work is the first to identify the zebrafish choroid plexus and to characterize its epithelial and vasculature integration. This study, in the context of other comparative anatomical studies, strongly indicates a conserved mechanism for development of the CP. Finally, we characterize a requirement for Notch signaling in the developing CP. This establishes the zebrafish CP as an important new system for the determination of key signaling pathways in the formation of this essential component of the vertebrate brain.
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