PUBLICATION
Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates
- Authors
- Chun, C.Z., Kaur, S., Samant, G.V., Wang, L., Pramanik, K., Garnaas, M.K., Li, K., Field, L., Mukhopadhyay, D., and Ramchandran, R.
- ID
- ZDB-PUB-080902-5
- Date
- 2009
- Source
- Blood 113(5): 1192-1199 (Journal)
- Registered Authors
- Field, Lyndsay, Garnaas, Maija, Ramchandran, Ramani
- Keywords
- none
- Datasets
- GEO:GSE12516
- MeSH Terms
-
- Animals
- Arteries/embryology*
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Cell Movement/physiology
- Endothelial Cells/enzymology
- Gene Expression Regulation, Developmental/physiology*
- Gene Expression Regulation, Enzymologic/physiology*
- Gene Knockdown Techniques
- Humans
- Oligonucleotide Array Sequence Analysis
- Protein Serine-Threonine Kinases/biosynthesis*
- Protein Serine-Threonine Kinases/genetics
- Receptors, Notch/genetics
- Receptors, Notch/metabolism
- Veins/embryology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 18723694 Full text @ Blood
Citation
Chun, C.Z., Kaur, S., Samant, G.V., Wang, L., Pramanik, K., Garnaas, M.K., Li, K., Field, L., Mukhopadhyay, D., and Ramchandran, R. (2009) Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates. Blood. 113(5):1192-1199.
Abstract
In vertebrates, molecular mechanisms dictate angioblasts' migration and subsequent differentiation into arteries and veins. In this study, we used a microarray screen to identify a novel member of the sucrose non-fermenting related kinase (snrk-1) family of serine/threonine kinases expressed specifically in the embryonic zebrafish vasculature and investigated its function in vivo. Using gain and loss of function studies in vivo, we show that Snrk-1 plays an essential role in the migration, maintenance and differentiation of angioblasts. The kinase function of Snrk-1 is critical for migration and maintenance, but not for the differentiation of angioblasts. In vitro, snrk-1 knockdown endothelial cells show only defects in migration. The snrk-1 gene acts downstream or parallel to notch and upstream of gridlock during artery-vein specification, and the human gene compensates for zebrafish snrk-1 knockdown, suggesting evolutionary conservation of function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping