PUBLICATION

Multiple regulatory elements mediating neuronal-specific expression of zebrafish sodium channel gene, Scn8aa

Authors
Wu, S.H., Chen, Y.H., Huang, F.L., Chang, C.H., Chang, Y.F., and Tsay, H.J.
ID
ZDB-PUB-080902-13
Date
2008
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   237(9): 2554-2565 (Journal)
Registered Authors
Chen, Yau-Hung, Tsay, Huey-Jen
Keywords
zebrafish, scn8aa, GFP, transgenic, neuronal-specific
MeSH Terms
  • Sodium Channels/genetics
  • Sodium Channels/metabolism*
  • Sodium Channels/physiology
  • Computational Biology
  • Binding Sites/genetics
  • NAV1.6 Voltage-Gated Sodium Channel
  • Neurons/metabolism*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Gene Expression Regulation, Developmental
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zebrafish Proteins/physiology
  • Immunohistochemistry
  • Base Sequence
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Regulatory Sequences, Nucleic Acid/genetics
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Animals
(all 25)
PubMed
18729213 Full text @ Dev. Dyn.
Abstract
Zebrafish scn8aa sodium channels mediate the majority of sodium conductance, which is essential for the embryonic locomotor activities. Here, we investigated the transcriptional regulation of scn8aa in developing zebrafish embryos by constructing a GFP reporter driven by a 15-kb fragment of scn8aa gene designed as scn8aa:GFP. GFP expression patterns of scn8aa:GFP temporally and spatially recapitulated the expression of endogenous scn8aa mRNA during zebrafish embryonic development, with one exception in the inner nuclear layer of the retina. Three novel elements, along with an evolutionarily conserved element shared with mouse SCN8A, modulated neuronal-specific expression of scn8aa. The deletion of each positive element reduced the expression levels in neurons without inducing ectopic GFP expression in non-neuronal cells. Our results demonstrate that these four regulatory elements function cooperatively to enhance scn8aa expression in the zebrafish nervous system.
Genes / Markers
Figures
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Expression
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
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    Fish
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    Orthology
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    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
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    Citation
    Wu, S.H., Chen, Y.H., Huang, F.L., Chang, C.H., Chang, Y.F., and Tsay, H.J. (2008) Multiple regulatory elements mediating neuronal-specific expression of zebrafish sodium channel gene, Scn8aa. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(9):2554-2565.