PUBLICATION
CLOCK is required for maintaining the circadian rhythms of opsin mRNA expression in photoreceptor cells
- Authors
- Li, P., Chaurasia, S.S., Gao, Y., Carr, A.L., Iuvone, P.M., and Li, L.
- ID
- ZDB-PUB-080825-32
- Date
- 2008
- Source
- The Journal of biological chemistry 283(46): 31673-31678 (Journal)
- Registered Authors
- Li, Lei
- Keywords
- none
- MeSH Terms
-
- Animals
- CLOCK Proteins
- Circadian Rhythm/physiology*
- Cyclic AMP/metabolism
- Gene Expression Regulation/genetics*
- Photoreceptor Cells/metabolism*
- RNA, Messenger/genetics
- Rod Opsins/genetics
- Rod Opsins/metabolism*
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 18687681 Full text @ J. Biol. Chem.
Citation
Li, P., Chaurasia, S.S., Gao, Y., Carr, A.L., Iuvone, P.M., and Li, L. (2008) CLOCK is required for maintaining the circadian rhythms of opsin mRNA expression in photoreceptor cells. The Journal of biological chemistry. 283(46):31673-31678.
Abstract
In zebrafish, the expression of long-wavelength cone (LC) opsin mRNA fluctuated rhythmically between the day and night. In a 24-hour period, expression was high in the afternoon and low in the early morning. This pattern of fluctuation persisted in zebrafish that were kept in constant darkness, suggesting an involvement of circadian clocks. Functional expression of Clock, a circadian clock gene that contributes to the central circadian pacemaker, was found to play an important role in maintaining the circadian rhythms of LC opsin mRNA expression. In zebrafish embryos in which the translation of Clock was inhibited by anti-Clock morpholinos (MOs), the circadian rhythms of LC opsin mRNA expression diminished. CLOCK may regulate the circadian rhythms of LC opsin mRNA expression via cyclic adenosine monophosphate (cAMP)-dependent signaling pathways. In control retinas, the concentration of cAMP was high in the early morning and low in the reminder of the day and night. Inhibition of Clock translation abolished the fluctuation in the concentration of cAMP, thereby diminishing the circadian rhythms of opsin mRNA expression. Transient increase of cAMP concentrations in the early morning (i.e., by treating the embryos with 8-Br-cAMP) restored the circadian rhythms of LC opsin mRNA expression in MO-treated embryos. Together, the data suggest that Clock plays important roles in regulating the circadian rhythms in photoreceptor cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping