PUBLICATION

Zebrafish endzone regulates neural crest-derived chromatophore differentiation and morphology

Authors

Arduini, B.L., Gallagher, G.R., and Henion, P.D.

ID

ZDB-PUB-080801-14

Date

2008

Source

PLoS One 3(7): e2845 (Journal)

Registered Authors

Arduini, Brigitte, Gallagher, Glen, Henion, Paul

Keywords

Melanophores, Chromatophores, Embryos, Zebrafish, Neural crest, Larvae, Cell differentiation, Neurons

MeSH Terms

Melanophores/metabolism*
Body Patterning
Phenotype
Time Factors
Models, Genetic
Models, Biological
Chromatophores/metabolism*
Chromosome Mapping
Cell Size
Mutation
Cell Differentiation*
Zebrafish
Animals
Neural Crest/metabolism

(all 14)

PubMed

18665240 Full text @ PLoS One

Abstract

The development of neural crest-derived pigment cells has been studied extensively as a model for cellular differentiation, disease and environmental adaptation. Neural crest-derived chromatophores in the zebrafish (Danio rerio) consist of three types: melanophores, xanthophores and iridiphores. We have identified the zebrafish mutant endzone (enz), that was isolated in a screen for mutants with neural crest development phenotypes, based on an abnormal melanophore pattern. We have found that although wild-type numbers of chromatophore precursors are generated in the first day of development and migrate normally in enz mutants, the numbers of all three chromatophore cell types that ultimately develop are reduced. Further, differentiated melanophores and xanthophores subsequently lose dendricity, and iridiphores are reduced in size. We demonstrate that enz function is required cell autonomously by melanophores and that the enz locus is located on chromosome 7. In addition, zebrafish enz appears to selectively regulate chromatophore development within the neural crest lineage since all other major derivatives develop normally. Our results suggest that enz is required relatively late in the development of all three embryonic chromatophore types and is normally necessary for terminal differentiation and the maintenance of cell size and morphology. Thus, although developmental regulation of different chromatophore sublineages in zebrafish is in part genetically distinct, enz provides an example of a common regulator of neural crest-derived chromatophore differentiation and morphology.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
aox5	enz^b431 (AB)	standard conditions	Prim-5	pigment cell	ISH	Fig. 4
aox5	WT	standard conditions	Prim-5	pigment cell	ISH	Fig. 4
crestin	enz^b431 (AB)	standard conditions	Prim-5	neural crest	ISH	Fig. 4
crestin	WT	standard conditions	Prim-5	neural crest	ISH	Fig. 4
csf1ra	enz^b431 (AB)	standard conditions	Long-pec	xanthophore	ISH	Fig. 6
csf1ra	WT	standard conditions	Long-pec	xanthophore	ISH	Fig. 6
dct	enz^b431 (AB)	standard conditions	Prim-5	melanoblast	ISH	Fig. 4
dct	enz^os18/os18	standard conditions	Prim-25	melanocyte	ISH	Fig. 5
dct	WT	standard conditions	Prim-5	melanoblast	ISH	Fig. 4
dct	WT	standard conditions	Prim-25	melanocyte	ISH	Fig. 5

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
enz^b431 (AB)	standard conditions	Prim-5	melanocyte morphology, normal	Fig. 5
enz^b431 (AB)	standard conditions	Prim-5	melanocyte size, normal	Fig. 5
enz^b431 (AB)	standard conditions	Prim-5	pigmentation disrupted, abnormal	Fig. 5
enz^b431 (AB)	standard conditions	Prim-15	melanocyte decreased amount, abnormal	Fig. 5
enz^b431 (AB)	standard conditions	Prim-15	melanocyte punctiform, abnormal	Fig. 5
enz^b431 (AB)	standard conditions	Prim-15	pigmentation disrupted, abnormal	Fig. 5
enz^b431 (AB)	standard conditions	Long-pec	iridophore decreased amount, abnormal	Fig. 1
enz^b431 (AB)	standard conditions	Long-pec	melanocyte decreased amount, abnormal	Fig. 1
enz^b431 (AB)	standard conditions	Long-pec	melanocyte punctiform, abnormal	Fig. 1
enz^b431 (AB)	standard conditions	Long-pec	neural crest cell morphology, normal	text only

1 - 10 of 49

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
b1	Point Mutation	slc24a5
b431	Unknown	enz
os7	Unknown	enz
os15	Unknown	enz
os18	Unknown	enz

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
enz^b431 (AB)
enz^os7/os7
enz^os15/os15
enz^os18/os18
slc24a5^b1/b1
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Arduini et al., 2008 ZDB-PUB-080801-14 PMID:18665240

Zebrafish endzone regulates neural crest-derived chromatophore differentiation and morphology

Arduini et al., 2008

ZDB-PUB-080801-14
PMID:18665240