PUBLICATION

Dynamic Fgf signaling couples morphogenesis and migration in the zebrafish lateral line primordium

Authors

Lecaudey, V., Cakan-Akdogan, G., Norton, W.H., and Gilmour, D.

ID

ZDB-PUB-080707-9

Date

2008

Source

Development (Cambridge, England) 135(16): 2695-2705 (Journal)

Registered Authors

Gilmour, Darren, Lecaudey, Virginie, Norton, Will

Keywords

Lateral line primordium, Zebrafish, Collective migration, Fgf, Rosette morphogenesis

MeSH Terms

Embryo, Nonmammalian/physiology
Mesoderm/cytology*
Mesoderm/physiology
Zebrafish Proteins/physiology*
Body Patterning/physiology
Epithelium/physiology
Morphogenesis/physiology
Cell Movement/physiology
Signal Transduction
Fibroblast Growth Factor 10/physiology*
Animals
Fibroblast Growth Factor 3/physiology*
Cell Differentiation/physiology
Zebrafish/embryology*
Zebrafish/physiology

(all 15)

PubMed

18599504 Full text @ Development

Abstract

The collective migration of cells in the form of cohesive tissues is a hallmark of both morphogenesis and repair. The extrinsic cues that direct these complex migrations usually act by regulating the dynamics of a specific subset of cells, those at the leading edge. Given that normally the function of tissue migration is to lay down multicellular structures, such as branched epithelial networks or sensory organs, it is surprising how little is known about the mechanisms that organize cells behind the leading edge. Cells of the zebrafish lateral line primordium switch from mesenchyme-like leader cells to epithelial rosettes that develop into mechanosensory organs. Here, we show that this transition is regulated by an Fgf signaling circuit that is active within the migrating primordium. Point sources of Fgf ligand drive surrounding cells towards a 'non-leader' fate by increasing their epithelial character, a prerequisite for rosette formation. We demonstrate that the dynamic expression of Fgf ligands determines the spatiotemporal pattern of epithelialization underlying sensory organ formation in the lateral line. Furthermore, this work uncovers a surprising link between internal tissue organization and collective migration.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
cxcr4b^{t26035/t26035}; zf106Tg	chemical treatment: SU5402	Prim-15 to Prim-25	posterior lateral line neuromast development disrupted, abnormal	Fig. 5
fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; zf106Tg	standard conditions	Prim-5 to Prim-25	posterior lateral line neuromast development disrupted, abnormal	Fig. S1
fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; zf106Tg	standard conditions	High-pec	posterior lateral line neuromast primordium migration rate, abnormal	Fig. 2
fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; zf106Tg	standard conditions	High-pec	posterior lateral line neuromast primordium migration variability of rate, abnormal	Fig. 4
fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; zf106Tg	standard conditions	High-pec	posterior lateral line primordium increased length, abnormal	Fig. 2
zf106Tg	chemical treatment: SU5402	Prim-25	establishment or maintenance of cell polarity disrupted, abnormal	Fig. 7
zf106Tg	chemical treatment: SU5402	Prim-25	posterior lateral line neuromast development disrupted, abnormal	Fig. 7
zf106Tg	chemical treatment: SU5402	Prim-25	posterior lateral line primordium decreased height, abnormal	Fig. 7
zf106Tg	chemical treatment: SU5402	High-pec	posterior lateral line neuromast primordium migration rate, abnormal	Fig. 2
zf106Tg	chemical treatment: SU5402	High-pec	posterior lateral line primordium increased length, abnormal	Fig. 2

1 - 10 of 14

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
pd1Tg	Tg(hsp70l:dnfgfr1a-EGFP)	Transgenic Insertion
t24149		Point Mutation	fgf3
t26035		Point Mutation	cxcr4b
tbvbo		Point Mutation	fgf10a
zf106Tg	Tg(-8.0cldnb:LY-EGFP)	Transgenic Insertion
zf115Tg	Tg(hsp70l:fgf3-MYC)	Transgenic Insertion

1 - 6 of 6

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
atoh1a	MO4-atoh1a	MRPHLNO

Fish

Fish
cxcr4b^{t26035/t26035}; zf106Tg
fgf3^{t24149/t24149}; zf106Tg
fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; zf106Tg
fgf10a^tbvbo/tbvbo; zf106Tg
pd1Tg
WT
WT + MO4-atoh1a
zf106Tg
zf106Tg + MO4-atoh1a
zf106Tg; zf115Tg

1 - 10 of 11

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Lecaudey et al., 2008 ZDB-PUB-080707-9 PMID:18599504

Dynamic Fgf signaling couples morphogenesis and migration in the zebrafish lateral line primordium

Lecaudey et al., 2008

ZDB-PUB-080707-9
PMID:18599504