PUBLICATION

Neuregulin-mediated ErbB3 signaling is required for formation of zebrafish dorsal root ganglion neurons

Authors

Honjo, Y., Kniss, J., and Eisen, J.S.

ID

ZDB-PUB-080707-10

Date

2008

Source

Development (Cambridge, England) 135(15): 2615-2625 (Journal)

Registered Authors

Eisen, Judith S., Honjo, Yasuko

Keywords

ErbB2, ErbB3, Neuregulin, Dorsal root ganglion, Neural crest migration, Zebrafish

MeSH Terms

Animals
Ganglia, Spinal/cytology
Ganglia, Spinal/embryology*
Ganglia, Spinal/metabolism*
Animals, Genetically Modified
Neuregulin-1/genetics
Neuregulin-1/metabolism*
Zebrafish/embryology
Zebrafish/genetics
Zebrafish/metabolism*
Gene Expression Regulation, Developmental
Signal Transduction
Mutation/genetics
Neurons/cytology
Neurons/metabolism*
Receptor, ErbB-3/genetics
Receptor, ErbB-3/metabolism*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Cell Movement
Neuregulins/genetics
Neuregulins/metabolism*
Receptor, ErbB-2/genetics
Receptor, ErbB-2/metabolism

(all 24)

PubMed

18599505 Full text @ Development

Abstract

Dorsal root ganglia (DRGs) arise from trunk neural crest cells that emerge from the dorsal neuroepithelium and coalesce into segmental streams that migrate ventrally along the developing somites. Proper formation of DRGs involves not only normal trunk neural crest migration, but also the ability of DRG progenitors to pause at a particular target location where they can receive DRG-promoting signals. In mammalian embryos, a receptor tyrosine kinase proto-oncogene, ErbB3, is required for proper trunk neural crest migration. Here, we show that in zebrafish mutants lacking ErbB3 function, neural crest cells do not pause at the location where DRGs normally form and DRG neurons are not generated. We also show that these mutants lack trunk neural crest-derived sympathetic neurons, but that cranial neural crest-derived enteric neurons appear normal. We isolated three genes encoding neuregulins, ErbB3 ligands, and show that two neuregulins function together in zebrafish trunk neural crest cell migration and in DRG formation. Together, our results suggest that ErbB3 signaling is required for normal migration of trunk, but not cranial, neural crest cells.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
crestin	erbb3b^st48/st48	standard conditions	Prim-5		neural crest cell	ISH	Fig. 2
crestin	erbb3b^st48/st48	standard conditions	26+ somites		neural crest cell	ISH	Fig. 2
crestin	WT	standard conditions	26+ somites		neural crest cell	ISH	Fig. 2
crestin	WT	standard conditions	Prim-5		neural crest cell	ISH	Fig. 2
crestin	WT	standard conditions	Prim-5		neural crest cell	ISH	Fig. 8
crestin	WT + MO1-nrg2a + MO2-nrg1	standard conditions	Prim-5		neural crest cell	ISH	Fig. 8
EGFP	ba2Tg/+	standard conditions	Prim-5		neural crest cell	IFL	Fig. 3
neurod1	erbb2^st61/st61	standard conditions	Prim-25	Not Detected	dorsal root ganglion	ISH	Fig. 1
neurod1	erbb2^st61/st61	standard conditions	Prim-25	Not Detected	neuron	ISH	Fig. 1
neurod1	erbb3b^st48/st48	standard conditions	Prim-25	Not Detected	dorsal root ganglion	ISH	Fig. 1

1 - 10 of 44

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-nrg2a	standard conditions	Day 4	dorsal root ganglion neuron decreased amount, abnormal	Fig. 7
WT + MO1-nrg2a	standard conditions	Day 4	dorsal root ganglion neuron mislocalised, abnormal	Fig. 7
WT + MO1-nrg2a	standard conditions	Day 4	peripheral nervous system development disrupted, abnormal	Fig. 7
WT + MO1-nrg2a + MO2-nrg1	standard conditions	Prim-5	neural crest cell migration disrupted, abnormal	Fig. 8
WT + MO1-nrg2a + MO2-nrg1	standard conditions	Prim-5	neural crest cell position, abnormal	Fig. 8
WT + MO1-nrg2a + MO2-nrg1	standard conditions	Day 4	dorsal root ganglion neuron absent, abnormal	Fig. 8
WT + MO2-nrg1	standard conditions	Long-pec	dorsal root ganglion neuron decreased amount, abnormal	Fig. 5
WT + MO2-nrg1	standard conditions	Long-pec	dorsal root ganglion neuron mislocalised, abnormal	Fig. 5
WT + MO2-nrg1	standard conditions	Long-pec	peripheral nervous system development disrupted, abnormal	Fig. 5
erbb2^st61/st61	standard conditions	Prim-15	dorsal root ganglion neuron absent, abnormal	Fig. 1

1 - 10 of 23

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ba2Tg	Tg(-4.9sox10:EGFP)	Transgenic Insertion
st48		Point Mutation	erbb3b
st61		Point Mutation	erbb2

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
nrg1	MO2-nrg1	MRPHLNO
nrg2a	MO1-nrg2a	MRPHLNO
nrg2b	MO1-nrg2b	MRPHLNO

Fish

Fish
ba2Tg/+
erbb2^st61/st61
erbb3b^st48/st48
erbb3b^st48/st48; ba2Tg/+
WT
WT + MO1-nrg2a
WT + MO1-nrg2a + MO2-nrg1
WT + MO1-nrg2b
WT + MO2-nrg1

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Honjo et al., 2008 ZDB-PUB-080707-10 PMID:18599505

Neuregulin-mediated ErbB3 signaling is required for formation of zebrafish dorsal root ganglion neurons

Honjo et al., 2008

ZDB-PUB-080707-10
PMID:18599505