PUBLICATION
Identification of a mutation in the Clock1 gene affecting zebrafish circadian rhythms
- Authors
- Tan, Y., DeBruyne, J., Cahill, G.M., and Wells, D.E.
- ID
- ZDB-PUB-080630-2
- Date
- 2008
- Source
- Journal of neurogenetics 22(2): 149-166 (Journal)
- Registered Authors
- Cahill, Greg
- Keywords
- Danio rerio, ENU mutagenesis, Locomotor rhymthicity, Melatonin cycles, Radiation hybrid mapping, Positional cloning
- MeSH Terms
-
- Amino Acid Substitution/genetics
- Animals
- CLOCK Proteins
- Chromosome Mapping
- Circadian Rhythm/genetics*
- DNA-Binding Proteins/genetics*
- Genetic Linkage
- Genotype
- Male
- Melatonin/metabolism
- Phenotype
- Pineal Gland/metabolism
- Point Mutation/genetics*
- Polymorphism, Genetic/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Trans-Activators/genetics*
- Zebrafish/genetics*
- PubMed
- 18569451 Full text @ J. Neurogenet.
Citation
Tan, Y., DeBruyne, J., Cahill, G.M., and Wells, D.E. (2008) Identification of a mutation in the Clock1 gene affecting zebrafish circadian rhythms. Journal of neurogenetics. 22(2):149-166.
Abstract
As part of an ongoing program to identify genes involved in maintaining circadian rhythms of zebrafish, 6,500 mutagenized genomes were screened for dominant mutants affecting circadian locomotor activity. Molecular analysis of one of these mutant lines, Clk1(dg3), revealed an I254N mutation in the PAS domain of the Clock1 protein. This isoleucine is tightly conserved in the Clock genes of several different species, and the I254N was not seen in any of the wild-type zebrafish population tested. Analysis of circadian activity rhythms as well as melatonin rhythms in homozygotes revealed the biological clock runs with a shortened period. The effect of this Clock1 mutation was characterized in vitro using a cell culture system where it appears to enhance the transactivation ability of the I254N Clock1 protein compared with that of the normal gene product.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping