PUBLICATION
Use of zebrafish models for the analysis of human disease
- Authors
- Zhu, H., and Zon, L.I.
- ID
- ZDB-PUB-080429-2
- Date
- 2002
- Source
- Current protocols in human genetics Chapter 15: Unit 15.3 (Chapter)
- Registered Authors
- Zon, Leonard I.
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cation Transport Proteins/genetics
- Cation Transport Proteins/metabolism
- Disease Models, Animal*
- Gene Targeting
- Genetic Engineering/methods
- Genetic Testing
- Genetics, Medical
- Humans
- Iron/metabolism
- Mutagenesis
- Oligonucleotides, Antisense/genetics
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 18428328 Full text @ Curr. Protoc. Hum. Genet.
Citation
Zhu, H., and Zon, L.I. (2002) Use of zebrafish models for the analysis of human disease. Current protocols in human genetics. Chapter 15:Unit 15.3.
Abstract
The zebrafish has emerged as a powerful animal model for human diseases. While it has long informed us about the biology of early development, it has recently come into favor for the investigation of clinically relevant problems. Genes conserved from fish to humans can be rapidly analyzed using the zebrafish embryo in what is essentially a transparent in vivo assay. This unit describes methodologies including genetic screening, targeted knockdowns, ectopic overexpression, and transgenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping