PUBLICATION
Zebrafish Neuroglobin Is a Cell-Membrane-Penetrating Globin
- Authors
- Watanabe, S., and Wakasugi, K.
- ID
- ZDB-PUB-080422-1
- Date
- 2008
- Source
- Biochemistry 47(19): 5266-5270 (Journal)
- Registered Authors
- Watanabe, Sumiko
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Apoptosis
- Cell Hypoxia
- Cell Line
- Cell Membrane Permeability*
- Conserved Sequence
- Globins/chemistry
- Globins/genetics
- Globins/metabolism*
- Humans
- Microscopy, Fluorescence
- Molecular Sequence Data
- Nerve Tissue Proteins/chemistry
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Oxidative Stress
- Oxygen/metabolism
- Protein Transport
- Rats
- Sequence Alignment
- Zebrafish/metabolism*
- PubMed
- 18416560 Full text @ Biochemistry
Citation
Watanabe, S., and Wakasugi, K. (2008) Zebrafish Neuroglobin Is a Cell-Membrane-Penetrating Globin. Biochemistry. 47(19):5266-5270.
Abstract
Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit of heterotrimeric G proteins (Galpha i) and acts as a guanine nucleotide dissociation inhibitor (GDI) for Galpha i. Recently, we used a protein delivery reagent, Chariot, and demonstrated that the GDI activity of human Ngb is tightly correlated with its neuroprotective activity. In the present study, we found that chimeric ZHHH Ngb, in which module M1 of human Ngb is replaced by that of zebrafish Ngb, protects PC12 cells against oxidative stress-induced cell death even in the absence of Chariot. Using fluorescein isothiocyanate (FITC)-labeled Ngb proteins, we demonstrated that both zebrafish and chimeric ZHHH Ngb can penetrate cell membranes in the absence of Chariot, suggesting that module M1 of zebrafish Ngb can translocate into cells. This is the first report of a native cell-membrane-penetrating globin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping