elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8
- Omori, Y., Zhao, C., Saras, A., Mukhopadhyay, S., Kim, W., Furukawa, T., Sengupta, P., Veraksa, A., and Malicki, J.
- Nature cell biology 10(4): 437-444 (Journal)
- Registered Authors
- Malicki, Jarema, Omori, Yoshihiro
- MeSH Terms
- Caenorhabditis elegans*/cytology
- Caenorhabditis elegans*/metabolism
- Carrier Proteins/genetics
- Carrier Proteins/metabolism*
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/metabolism*
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Two-Hybrid System Techniques
- Vesicular Transport Proteins/genetics
- Vesicular Transport Proteins/metabolism
- Zebrafish*/anatomy & histology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- rab GTP-Binding Proteins/genetics
- rab GTP-Binding Proteins/metabolism*
- 18364699 Full text @ Nat. Cell Biol.
Omori, Y., Zhao, C., Saras, A., Mukhopadhyay, S., Kim, W., Furukawa, T., Sengupta, P., Veraksa, A., and Malicki, J. (2008) elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8. Nature cell biology. 10(4):437-444.
The formation and function of cilia involves the movement of intraflagellar transport (IFT) particles underneath the ciliary membrane, along axonemal microtubules. Although this process has been studied extensively, its molecular basis remains incompletely understood. For example, it is unknown how the IFT particle interacts with transmembrane proteins. To study the IFT particle further, we examined elipsa, a locus characterized by mutations that cause particularly early ciliogenesis defects in zebrafish. We show here that elipsa encodes a coiled-coil polypeptide that localizes to cilia. Elipsa protein binds to Ift20, a component of IFT particles, and Elipsa homologue in Caenorhabditis elegans, DYF-11, translocates in sensory cilia, similarly to the IFT particle. This indicates that Elipsa is an IFT particle polypeptide. In the context of zebrafish embryogenesis, Elipsa interacts genetically with Rabaptin5, a well-studied regulator of endocytosis, which in turn interacts with Rab8, a small GTPase, known to localize to cilia. We show that Rabaptin5 binds to both Elipsa and Rab8, suggesting that these proteins provide a bridging mechanism between the IFT particle and protein complexes that assemble at the ciliary membrane.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes