PUBLICATION
Identification and characterization of zebrafish ocular formation genes
- Authors
- Wang, H., Kesinger, J.W., Zhou, Q., Wren, J.D., Martin, G., Turner, S., Tang, Y., Frank, M.B., and Centola, M.
- ID
- ZDB-PUB-080327-8
- Date
- 2008
- Source
- Genome 51(3): 222-235 (Journal)
- Registered Authors
- Wang, Han
- Keywords
- masterblind/axin1, retina, lens, opsin, crystallin, bzw1a, atp6v0c, hspa8, rpl7a, oligonucleotide microarrays, in situ hybridization
- MeSH Terms
-
- Animals
- Axin Protein
- Down-Regulation
- Embryo, Nonmammalian
- Eye/metabolism*
- Eye Proteins/genetics*
- Gene Expression Regulation, Developmental
- Gene Regulatory Networks
- In Situ Hybridization
- Phylogeny
- Repressor Proteins/genetics
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 18356958 Full text @ Genome
Citation
Wang, H., Kesinger, J.W., Zhou, Q., Wren, J.D., Martin, G., Turner, S., Tang, Y., Frank, M.B., and Centola, M. (2008) Identification and characterization of zebrafish ocular formation genes. Genome. 51(3):222-235.
Abstract
To study genes that are specifically expressed in the eyes, we employed microarray and in situ hybridization analyses to identify and characterize differentially expressed ocular genes in eyeless masterblind (mbl−/−) zebrafish (Danio rerio). Among 70 differentially expressed genes in the mbl-/- mutant identified by microarray analysis, 8 down-regulated genes were characterized, including 4 eye-specific genes, opsin 1 short-wave-sensitive 1 (opn1sw1), crystallinssa1b (cryba1b), crystallinssa2b (cryba2b), and crystallinγ M2d3 (crygm2d3); 2 eye and brain genes, ATPase, H+ transporting, lysosomal, V0 subunit c (atp6v0c) and basic leucine zipper and W2 domains 1a (bzw1a)nssa1b (cryba1b), crystallinssa2b (cryba2b), and crystallinγ M2d3 (crygm2d3); 2 eye and brain genes, ATPase, H+ tra; and 2 constitutive genes, heat shock protein 8 (hspa8) and ribosomal protein L7a (rpl7a). In situ hybridization experiments confirmed down-regulation of these 8 ocular formation genes in mbl-/- zebrafish and showed their ocular and dynamic temporal expression patterns during zebrafish early development. Further, an automated literature analysis of the 70 differentially expressed genes identified a sub-network of genes with known associations, either with each other or with ocular structures or development, and shows how this study contributes to the current body of knowledge.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping