PUBLICATION

Zebrafish sip1a and sip1b are essential for normal axial and neural patterning

Authors
Delalande, J.M., Guyote, M.E., Smith, C.M., and Shepherd, I.T.
ID
ZDB-PUB-080327-2
Date
2008
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   237(4): 1060-1069 (Journal)
Registered Authors
Shepherd, Iain T.
Keywords
zebrafish, enteric nervous system, SIP1, Mowat-Wilson syndrome, neural crest
MeSH Terms
  • Animals
  • Base Sequence
  • Body Patterning*
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Morphogenesis
  • Neural Crest/cytology
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Phenotype
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism*
  • Stem Cells/physiology
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
18351671 Full text @ Dev. Dyn.
Abstract
Smad-interacting protein-1 (SIP1) has been implicated in the development of Mowat-Wilson syndrome whose patients exhibit Hirschsprung disease, an aganglionosis of the large intestine, as well as other phenotypes. We have identified and cloned two sip1 orthologues in zebrafish. Both sip1 orthologues are expressed maternally and have dynamic zygotic expression patterns that are temporally and spatially distinct. We have investigated the function of both orthologues using translation and splice-blocking morpholino antisense oligonucleotides. Knockdown of the orthologues causes axial and neural patterning defects consistent with the previously described function of SIP1 as an inhibitor of BMP signaling. In addition, knockdown of both genes leads to a significant reduction/loss of the post-otic cranial neural crest. This results in a subsequent absence of neural crest precursors in the posterior pharyngeal arches and a loss of enteric precursors in the intestine.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping