PUBLICATION

Suppression of the endoplasmic reticulum calcium pump during zebrafish gastrulation affects left-right asymmetry of the heart and brain

Authors

Kreiling, J.A., Balantac, Z.L., Crawford, A.R., Ren, Y., Toure, J., Zchut, S., Kochilas, L., and Creton, R.

ID

ZDB-PUB-080327-14

Date

2008

Source

Mechanisms of Development 125(5-6): 396-410 (Journal)

Registered Authors

Creton, Robbert

Keywords

Calcium, Endoplasmic reticulum, Signaling, Gastrulation, Danio rerio, ER

MeSH Terms

Gastrula/physiology*
Zebrafish Proteins/biosynthesis*
Zebrafish Proteins/physiology
Models, Biological
Zebrafish
Body Patterning
Brain/embryology*
In Situ Hybridization
Animals
Heart/embryology*
Gene Expression Regulation, Developmental
Dyneins/biosynthesis*
Dyneins/physiology
Calcium/metabolism
Calcium/physiology*
T-Box Domain Proteins/biosynthesis*
T-Box Domain Proteins/physiology
Thapsigargin/pharmacology
Sodium-Calcium Exchanger/biosynthesis*
Sodium-Calcium Exchanger/physiology
Fetal Proteins
Endoplasmic Reticulum/metabolism*

(all 22)

PubMed

18359204 Full text @ Mech. Dev.

Abstract

Vertebrate embryos generate striking Ca(2+) patterns, which are unique regulators of dynamic developmental events. In the present study, we used zebrafish embryos as a model system to examine the developmental roles of Ca(2+) during gastrulation. We found that gastrula stage embryos maintain a distinct pattern of cytosolic Ca(2+) along the dorsal-ventral axis, with higher Ca(2+) concentrations in the ventral margin and lower Ca(2+) concentrations in the dorsal margin and dorsal forerunner cells. Suppression of the endoplasmic reticulum Ca(2+) pump with 0.5muM thapsigargin elevates cytosolic Ca(2+) in all embryonic regions and induces a randomization of laterality in the heart and brain. Affected hearts, visualorsal forerunner cells. Suppression of the endoplasmic reticulum Ca(2+) pump with 0.5muM thapsigargin elevates cytosolic Ca(2+) in all emized in living embryos by a subtractive imaging technique, displayed either a reversal or loss of left-right asymmetry. Brain defects include a left-right reversal of pitx2 expression in the dorsal diencephalon and a left-right reversal of the prominent habenular nucleus in the brain. Embryos are sensitive to inhibition of the endoplasmic reticulum Ca(2+) pump during early and mid gastrulation and lose their sensitivity during late gastrulation and early segmentation. Suppression of the endoplasmic reticulum Ca(2+) pump during gastrulation inhibits expression of no tail (ntl) and left-right dynein related (lrdr) in the dorsal forerunner cells and affects development of Kupffer's vesicle, a ciliated organ that generates a counter-clockwise flow of fluid. Previous studies have shown that Ca(2+) plays a role in Kupffer's vesicle function, influencing ciliary motility and translating the vesicle's counter-clockwise flow into asymmetric patterns of gene expression. The present results suggest that Ca(2+) plays an additional role in the formation of Kupffer's vesicle.

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