PUBLICATION

Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone

Authors
Hoffmann, J.L., Thomason, R.G., Lee, D.M., Brill, J.L., Price, B.B., Carr, G.J., and Versteeg, D.J.
ID
ZDB-PUB-080326-14
Date
2008
Source
Aquatic toxicology (Amsterdam, Netherlands)   87(2): 69-80 (Journal)
Registered Authors
Lee, Debbie M.
Keywords
Zebrafish, Methyldihydrotestosterone, Microarray, Liver, 17β-Estradiol, Testosterone, Vitellogenin, Gonadosomatic index
MeSH Terms
  • Animals
  • Vitellogenins/blood
  • Oligonucleotide Array Sequence Analysis*
  • Dihydrotestosterone/analogs & derivatives*
  • Dihydrotestosterone/toxicity
  • Testosterone/blood
  • Female
  • Environmental Exposure
  • Gene Expression Profiling*
  • Estradiol/blood
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/genetics*
  • Ovary/drug effects
  • Water Pollutants, Chemical/toxicity*
  • Liver/drug effects*
  • Liver/metabolism
  • Gene Expression Regulation/drug effects*
(all 17)
PubMed
18339436 Full text @ Aquat. Toxicol.
Abstract
Concentration and time-dependent changes in hepatic gene expression were examined in adult, female zebrafish (Danio rerio) exposed to 0, 0.1, 0.7, 4.9mug/L of a model androgen, 17alpha-methyldihydrotestosterone (MDHT). At 24 and 168h, fish were sacrificed and liver was extracted for gene expression analysis using custom Affymetrix GeneChip((R)) Zebrafish Genome Microarrays. In an effort to link gene expression changes to higher levels of biological organization, blood was collected for measurement of plasma steroid hormones (17beta-estradiol (E2), testosterone (T)) and vitellogenin (VTG) using ELISA. Body and ovary weight were also measured. A significant reduction in E2 occurred at 24h
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Hoffmann, J.L., Thomason, R.G., Lee, D.M., Brill, J.L., Price, B.B., Carr, G.J., and Versteeg, D.J. (2008) Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone. Aquatic toxicology (Amsterdam, Netherlands). 87(2):69-80.