PUBLICATION

Three-dimensional real-time imaging of cardiac cell motions in living embryos

Authors
Lu, J., Pereira, F., Fraser, S.E., and Gharib, M.
ID
ZDB-PUB-080309-27
Date
2008
Source
Journal of Biomedical Optics   13(1): 014006 (Journal)
Registered Authors
Fraser, Scott E.
Keywords
none
MeSH Terms
  • Animals
  • Cells, Cultured
  • Equipment Design
  • Equipment Failure Analysis
  • Image Interpretation, Computer-Assisted/instrumentation*
  • Image Interpretation, Computer-Assisted/methods
  • Imaging, Three-Dimensional/instrumentation*
  • Imaging, Three-Dimensional/methods
  • Microscopy, Video/instrumentation*
  • Microscopy, Video/methods
  • Myocytes, Cardiac/cytology*
  • Myocytes, Cardiac/physiology*
  • Sensitivity and Specificity
  • Zebrafish/anatomy & histology*
  • Zebrafish/embryology*
PubMed
18315364 Full text @ J. Biomed. Opt.
Abstract
While quantitative analysis of dynamic biological cell motions in vivo is of great biomedical interest, acquiring 3-D (plus time) information is difficult due to the lack of imaging tools with sufficient spatial and temporal resolution. A novel 3-D high-speed microscopic imaging system is developed to enable 3-D time series data acquisition, based on a defocusing technique (DDPIV). Depth coordinate Z is resolved by the triangular image patterns generated by a mask with three apertures forming an equilateral triangle. Application of this technique to microscale imaging is validated by calibration of targets spread over the image field. 1-mum fluorescent tracer particles are injected into the blood stream of 32 h post-fertilization developing zebrafish embryos to help describe cardiac cell motions. 3-D and velocity fields of cardiovascular blood flow and trajectories of heart-wall motions are obtained.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes