Three-dimensional real-time imaging of cardiac cell motions in living embryos
- Lu, J., Pereira, F., Fraser, S.E., and Gharib, M.
- Journal of Biomedical Optics 13(1): 014006 (Journal)
- Registered Authors
- Fraser, Scott E.
- MeSH Terms
- Cells, Cultured
- Equipment Design
- Equipment Failure Analysis
- Image Interpretation, Computer-Assisted/instrumentation*
- Image Interpretation, Computer-Assisted/methods
- Imaging, Three-Dimensional/instrumentation*
- Imaging, Three-Dimensional/methods
- Microscopy, Video/instrumentation*
- Microscopy, Video/methods
- Myocytes, Cardiac/cytology*
- Myocytes, Cardiac/physiology*
- Sensitivity and Specificity
- Zebrafish/anatomy & histology*
- 18315364 Full text @ J. Biomed. Opt.
Lu, J., Pereira, F., Fraser, S.E., and Gharib, M. (2008) Three-dimensional real-time imaging of cardiac cell motions in living embryos. Journal of Biomedical Optics. 13(1):014006.
While quantitative analysis of dynamic biological cell motions in vivo is of great biomedical interest, acquiring 3-D (plus time) information is difficult due to the lack of imaging tools with sufficient spatial and temporal resolution. A novel 3-D high-speed microscopic imaging system is developed to enable 3-D time series data acquisition, based on a defocusing technique (DDPIV). Depth coordinate Z is resolved by the triangular image patterns generated by a mask with three apertures forming an equilateral triangle. Application of this technique to microscale imaging is validated by calibration of targets spread over the image field. 1-mum fluorescent tracer particles are injected into the blood stream of 32 h post-fertilization developing zebrafish embryos to help describe cardiac cell motions. 3-D and velocity fields of cardiovascular blood flow and trajectories of heart-wall motions are obtained.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes