PUBLICATION

No tail co-operates with non-canonical Wnt signaling to regulate posterior body morphogenesis in zebrafish

Authors

Marlow, F., Gonzalez, E.M., Yin, C., Rojo, C., and Solnica-Krezel, L.

ID

ZDB-PUB-080309-1

Date

2004

Source

Development (Cambridge, England) 131(1): 203-216 (Journal)

Registered Authors

Gonzalez, Encina, Marlow, Florence, Solnica-Krezel, Lilianna, Yin, Chunyue

Keywords

Convergence, Extension, Gastrulation, knypek, silberblick (wnt11), pipetail (wnt5), subduction

MeSH Terms

Zebrafish/embryology*
Zebrafish/genetics
Proto-Oncogene Proteins/physiology*
Embryo, Nonmammalian/physiology*
Phenotype
Gastrula/physiology*
Genotype
Zebrafish Proteins*
Mutation
Signal Transduction
Animals
Tail/abnormalities
Tail/embryology*
Movement/physiology
Gene Expression Regulation, Developmental/genetics*
Body Patterning/physiology
Morphogenesis/physiology*
Wnt Proteins

(all 18)

PubMed

14660439 Full text @ Development

Abstract

The vertebrate posterior body is formed by a combination of the gastrulation movements that shape the head and anterior trunk and posterior specific cell behaviors. Here, we investigated whether genes that regulate cell movements during gastrulation [no tail (ntl)/brachyury, knypek (kny) and pipetail (ppt)/wnt5] interact to regulate posterior body morphogenesis. Both kny;ntl and ppt;ntl double mutant embryos exhibit synergistic trunk and tail shortening by early segmentation. Gene expression analysis in the compound mutants indicates that anteroposterior germ-layer patterning is largely normal and that the tail elongation defects are not due to failure to specify or maintain posterior tissues. Moreover, ntl interacts with ppt and kny to synergistically regulate the posterior expression of the gene encoding bone morphogenetic protein 4 (bmp4) but not of other known T-box genes, fibroblast growth factor genes or caudal genes. Examination of mitotic and apoptotic cells indicates that impaired tail elongation is not simply due to decreased cell proliferation or increased cell death. Cell tracing in ppt;ntl and kny;ntl mutants demonstrates that the ventral derived posterior tailbud progenitors move into the tailbud. However, gastrulation-like convergence and extension movements and cell movements within the posterior tailbud are impaired. Furthermore, subduction movements of cells into the mesendoderm are reduced in kny;ntl and ppt;ntl mutants. We propose that Ntl and the non-canonical Wnt pathway components Ppt and Kny function in parallel, partially redundant pathways to regulate posterior body development. Our work initiates the genetic dissection of posterior body morphogenesis and links genes to specific tail-forming movements. Moreover, we provide genetic evidence for the notion that tail development entails a continuation of mechanisms regulating gastrulation together with mechanisms unique to the posterior body.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
bmp4	gpc4^m119/m119	standard conditions	5-9 somites		prechordal plate	ISH	Fig. 5
bmp4	gpc4^m119/m119	standard conditions	5-9 somites		tail bud	ISH	Fig. 5
bmp4	gpc4^m119/m119; tbxta^b195/b195	standard conditions	5-9 somites		prechordal plate	ISH	Fig. 5
bmp4	gpc4^m119/m119; tbxta^b195/b195	standard conditions	5-9 somites	Not Detected	tail bud	ISH	Fig. 5
bmp4	tbxta^b195/b195	standard conditions	5-9 somites		prechordal plate	ISH	Fig. 5
bmp4	tbxta^b195/b195	standard conditions	5-9 somites		tail bud	ISH	Fig. 5
bmp4	wnt5b^ta98/ta98	standard conditions	5-9 somites		prechordal plate	ISH	Fig. 5
bmp4	wnt5b^ta98/ta98	standard conditions	5-9 somites		tail bud	ISH	Fig. 5
bmp4	wnt5b^ta98/ta98; tbxta^b195/b195	standard conditions	5-9 somites		prechordal plate	ISH	Fig. 5
bmp4	wnt5b^ta98/ta98; tbxta^b195/b195	standard conditions	5-9 somites	Not Detected	tail bud	ISH	Fig. 5

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
gpc4^m119/m119	standard conditions	5-9 somites	whole organism decreased length, abnormal	Fig. 3
gpc4^m119/m119	standard conditions	14-19 somites	extension aplastic, abnormal	Fig. 3
gpc4^m119/m119	standard conditions	14-19 somites	post-vent region decreased length, abnormal	Fig. 3
gpc4^m119/m119	standard conditions	Prim-5	extension decreased length, abnormal	Fig. 2
gpc4^m119/m119	standard conditions	Prim-5	post-vent region decreased length, abnormal	Fig. 2
gpc4^m119/m119; tbxta^b195/b195	standard conditions	5-9 somites	convergent extension involved in axis elongation disrupted, abnormal	Fig. 7
gpc4^m119/m119; tbxta^b195/b195	standard conditions	5-9 somites	convergent extension involved in axis elongation disrupted, abnormal	Fig. 8
gpc4^m119/m119; tbxta^b195/b195	standard conditions	5-9 somites	whole organism decreased length, abnormal	Fig. 3
gpc4^m119/m119; tbxta^b195/b195	standard conditions	14-19 somites	extension aplastic, abnormal	Fig. 3
gpc4^m119/m119; tbxta^b195/b195	standard conditions	14-19 somites	post-vent region decreased length, abnormal	Fig. 3

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
b195	Insertion	tbxta
m119	Point Mutation	gpc4
ta98	Point Mutation	wnt5b
tz216	Point Mutation	wnt11f2

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
gpc4^m119/m119
gpc4^m119/m119; tbxta^b195/b195
tbxta^b195/b195
wnt5b^ta98/ta98
wnt5b^ta98/ta98; tbxta^b195/b195
wnt11f2^tz216/tz216
wnt11f2^tz216/tz216; tbxta^b195/b195
wnt11f2^tz216/tz216; wnt5b^ta98/ta98
WT

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

Marlow et al., 2004 ZDB-PUB-080309-1 PMID:14660439

No tail co-operates with non-canonical Wnt signaling to regulate posterior body morphogenesis in zebrafish

Marlow et al., 2004

ZDB-PUB-080309-1
PMID:14660439