PUBLICATION

Interplay between Etsrp/ER71, scl and alk8 signaling controls endothelial and myeloid cell formation

Authors
Sumanas, S., Gomez, G., Zhao, Y., Park, C., Choi, K., and Lin, S.
ID
ZDB-PUB-080228-6
Date
2008
Source
Blood   111(9): 4500-4510 (Journal)
Registered Authors
Gomez, Gustavo, Lin, Shuo, Sumanas, Saulius, Zhao, Yan
Keywords
none
MeSH Terms
  • Activin Receptors, Type I/physiology*
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/physiology*
  • Embryo, Nonmammalian
  • Endothelial Cells/cytology*
  • Hematopoiesis
  • Humans
  • Mice
  • Myeloid Cells/cytology*
  • Proto-Oncogene Proteins/physiology*
  • Signal Transduction
  • Transcription Factors/physiology*
  • Zebrafish
  • Zebrafish Proteins/physiology*
PubMed
18270322 Full text @ Blood
Abstract
Vascular endothelial and myeloid cells have been proposed to originate from a common precursor cell, hemangioblast. Mechanism of endothelial and myeloid cell specification and differentiation is poorly understood. We have previously described the endothelial-specific zebrafish Ets1-related protein (Etsrp), which was both necessary and sufficient to initiate vasculogenesis in the zebrafish embryos. Here we identify human Etv2/ER71 and mouse ER71 proteins as functional orthologs of Etsrp. Overexpression of mouse ER71 and Etsrp caused strong expansion of hemangioblast and vascular endothelial lineages in a zebrafish embryo. In addition, we show that etsrp is also required for the formation of myeloid but not erythroid cells. In the absence of etsrp function, the number of granulocytes and macrophages is greatly reduced. Etsrp overexpression causes expansion of both myeloid and vascular endothelial lineages. Analysis of mosaic embryos indicates that etsrp functions cell-autonomously in inducing myeloid lineage. We further demonstrate that the choice of endothelial versus myeloid fate depends on combinatorial effect of etsrp, scl and alk8 genes.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping