PUBLICATION
Characterization and comparative expression of zebrafish calpain system genes during early development
- Authors
- Lepage, S.E., and Bruce, A.E.
- ID
- ZDB-PUB-080227-21
- Date
- 2008
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 237(3): 819-829 (Journal)
- Registered Authors
- Bruce, Ashley, Lepage, Stephanie
- Keywords
- calpain, calpastatin, zebrafish, capn1, capn2, capn4, capn8, capns1, capns2
- MeSH Terms
-
- Animals
- Calpain/genetics*
- Calpain/metabolism
- Catalytic Domain/genetics
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/enzymology*
- Evolution, Molecular
- Gene Expression Regulation, Developmental*
- Phylogeny
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 18265014 Full text @ Dev. Dyn.
Citation
Lepage, S.E., and Bruce, A.E. (2008) Characterization and comparative expression of zebrafish calpain system genes during early development. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(3):819-829.
Abstract
The classic calpain system has been implicated in regulating a variety of cellular processes including cell adhesion, migration, and intracellular signaling; however, little is known regarding the function of this system in vivo. Two heterodimeric Ca(2+)-dependent cysteine proteases, mu-calpain (CAPN1) and m-calpain (CAPN2), and the endogenous inhibitor calpastatin (CAST) comprise the classic/ubiquitous calpain system in mammals. Recently, knockout of two murine classic calpain genes, Capn2 and Capn4/Capns1, revealed that components of the classic system are indispensable for preimplantation development. We identified four classic calpain catalytic subunit genes (capn1a, 1b, 2a, 2b), two regulatory subunit genes (capns1a, 1b), and calpastatin (cast) from the zebrafish. Our data suggest that the components of the classic mammalian system are both conserved and expanded in the teleost lineage. In contrast to the classic/ubiquitous mammalian system, zebrafish calpain system genes acquire unique, tissue-specific patterns of expression within the first 2 days of development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping