PUBLICATION
Visualization and experimental analysis of blood vessel formation using transgenic zebrafish
- Authors
- Cha, Y.R., and Weinstein, B.M.
- ID
- ZDB-PUB-080226-11
- Date
- 2007
- Source
- Birth defects research. Part C, Embryo today : reviews 81(4): 286-296 (Review)
- Registered Authors
- Weinstein, Brant M.
- Keywords
- blood vessels, lymphatic vessels, confocal microangiography, vascular-specific transgenic zebrafish, multiphoton time-lapse microscopy
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blood Vessels/embryology*
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/genetics
- Lymphatic Vessels/embryology
- Microscopy, Fluorescence
- Mutation
- Neovascularization, Physiologic
- Recombinant Proteins/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 18228261 Full text @ Birth Defects Res. C Embryo Today
Citation
Cha, Y.R., and Weinstein, B.M. (2007) Visualization and experimental analysis of blood vessel formation using transgenic zebrafish. Birth defects research. Part C, Embryo today : reviews. 81(4):286-296.
Abstract
The mechanisms of blood vessel formation have become a subject of enormous scientific and clinical interest. However, it is difficult to visualize the developing vasculature in most living animals due to the ubiquitous and deep localization of vessels within other tissues. The establishment of vascular-specific transgenic zebrafish with fluorescently "tagged" blood vessels has facilitated high-resolution imaging studies of developing blood and lymphatic vessels in vivo. Use of these transgenic lines for genetic and chemical screening, experimental manipulations, and time-lapse imaging has extended our knowledge of how complex networks of vessels assemble in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping