PUBLICATION

Visualization and experimental analysis of blood vessel formation using transgenic zebrafish

Authors
Cha, Y.R., and Weinstein, B.M.
ID
ZDB-PUB-080226-11
Date
2007
Source
Birth defects research. Part C, Embryo today : reviews   81(4): 286-296 (Review)
Registered Authors
Weinstein, Brant M.
Keywords
blood vessels, lymphatic vessels, confocal microangiography, vascular-specific transgenic zebrafish, multiphoton time-lapse microscopy
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/embryology*
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/genetics
  • Lymphatic Vessels/embryology
  • Microscopy, Fluorescence
  • Mutation
  • Neovascularization, Physiologic
  • Recombinant Proteins/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
18228261 Full text @ Birth Defects Res. C Embryo Today
Abstract
The mechanisms of blood vessel formation have become a subject of enormous scientific and clinical interest. However, it is difficult to visualize the developing vasculature in most living animals due to the ubiquitous and deep localization of vessels within other tissues. The establishment of vascular-specific transgenic zebrafish with fluorescently "tagged" blood vessels has facilitated high-resolution imaging studies of developing blood and lymphatic vessels in vivo. Use of these transgenic lines for genetic and chemical screening, experimental manipulations, and time-lapse imaging has extended our knowledge of how complex networks of vessels assemble in vivo.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes