PUBLICATION

PTRF-Cavin, a Conserved Cytoplasmic Protein Required for Caveola Formation and Function

Authors
Hill, M.M., Bastiani, M., Luetterforst, R., Kirkham, M., Kirkham, A., Nixon, S.J., Walser, P., Abankwa, D., Oorschot, V.M., Martin, S., Hancock, J.F., and Parton, R.G.
ID
ZDB-PUB-080209-9
Date
2008
Source
Cell   132(1): 113-124 (Journal)
Registered Authors
Parton, Robert G.
Keywords
none
MeSH Terms
  • Animals
  • Bees
  • Caveolae/metabolism*
  • Caveolae/ultrastructure
  • Caveolin 1/metabolism
  • Cell Line
  • Cell Line, Tumor
  • Cell Membrane/metabolism*
  • Cell Membrane/ultrastructure
  • Cells, Cultured
  • Conserved Sequence
  • Cricetinae
  • Cytoplasm/metabolism*
  • Cytoplasm/ultrastructure
  • Evolution, Molecular
  • Fibroblasts
  • Golgi Apparatus/metabolism
  • Golgi Apparatus/ultrastructure
  • Intracellular Membranes/metabolism
  • Intracellular Membranes/ultrastructure
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism*
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • NIH 3T3 Cells
  • Notochord/embryology
  • Notochord/metabolism
  • Notochord/ultrastructure
  • RNA-Binding Proteins
  • Species Specificity
  • Zebrafish
PubMed
18191225 Full text @ Cell
Abstract
Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. We used comparative proteomics to identify PTRF (also called Cav-p60, Cavin) as a putative caveolar coat protein. PTRF-Cavin selectively associates with mature caveolae at the plasma membrane but not Golgi-localized caveolin. In prostate cancer PC3 cells, and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, and caveolin resides on flat plasma membrane. Expression of PTRF-Cavin in PC3 cells is sufficient to cause formation of caveolae. Knockdown of PTRF-Cavin reduces caveolae density, both in mammalian cells and in the zebrafish. Caveolin remains on the plasma membrane in PTRF-Cavin knockdown cells but exhibits increased lateral mobility and accelerated lysosomal degradation. We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping