PUBLICATION

colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis

Authors

Ignatius, M.S., Moose, H.E., El-Hodiri, H.M., and Henion, P.D.

ID

ZDB-PUB-071219-5

Date

2008

Source

Developmental Biology 313(2): 568-583 (Journal)

Registered Authors

Henion, Paul, Ignatius, Myron

Keywords

Neural crest, Melanophore, Histone deacetylase1, foxd3, mitfa, c-kit, Zebrafish

MeSH Terms

Models, Biological
Base Sequence
Binding Sites
Microinjections
Neural Crest/cytology
Neural Crest/embryology
Microphthalmia-Associated Transcription Factor/metabolism*
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Developmental*
Animals
In Situ Hybridization
Embryo, Nonmammalian
Promoter Regions, Genetic
Zebrafish/embryology
Zebrafish/genetics
Zebrafish/metabolism
Zebrafish/physiology*
Protein Binding
Histone Deacetylase 1
Mutation
Melanophores/cytology
Melanophores/metabolism
Melanophores/physiology
Forkhead Transcription Factors/metabolism*
Histone Deacetylases/genetics
Histone Deacetylases/metabolism
Histone Deacetylases/physiology*
Cell Movement
Molecular Sequence Data
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Zebrafish Proteins/physiology*
Oligonucleotides, Antisense/pharmacology

(all 33)

PubMed

18068699 Full text @ Dev. Biol.

Abstract

Neural crest-derived pigment cell development has been used extensively to study cell fate specification, migration, proliferation, survival and differentiation. Many of the genes and regulatory mechanisms required for pigment cell development are conserved across vertebrates. The zebrafish mutant colgate (col)/histone deacetylase1 (hdac1) has reduced numbers, delayed differentiation and decreased migration of neural crest-derived melanophores and their precursors. In hdac1(col) mutants normal numbers of premigratory neural crest cells are induced. Later, while there is only a slight reduction in the number of neural crest cells in hdac1(col) mutants, there is a severe reduction in the number of mitfa-positive melanoblasts suggesting that hdac1 is required for melanoblast specification. Concomitantly, there is a significant increase in and prolonged expression of foxd3 in neural crest cells in hdac1(col) mutants. We found that partially reducing Foxd3 expression in hdac1(col) mutants rescues mitfa expression and the melanophore defects in hdac1(col) mutants. Furthermore, we demonstrate the ability of Foxd3 to physically interact at the mitfa promoter. Because mitfa is required for melanoblast specification and development, our results suggest that hdac1 is normally required to suppress neural crest foxd3 expression thus de-repressing mitfa resulting in melanogenesis by a subset of neural crest-derived cells.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
aox5	hdac1^b382/b382	standard conditions	Prim-15	unspecified	ISH	Fig. S2
aox5	hdac1^b382/b382	standard conditions	Long-pec	xanthophore	ISH	Fig. 3
aox5	WT	standard conditions	Prim-15	unspecified	ISH	Fig. S2
aox5	WT	standard conditions	Long-pec	xanthophore	ISH	Fig. 3
crestin	hdac1^b382/b382	standard conditions	14-19 somites	trunk neural crest	ISH	Fig. 4
crestin	hdac1^b382/b382	standard conditions	Prim-5	trunk neural crest	ISH	Fig. 4
crestin	WT	standard conditions	Prim-5	trunk neural crest	ISH	Fig. 4
crestin	WT	standard conditions	14-19 somites	trunk neural crest	ISH	Fig. 4
csf1ra	hdac1^b382/b382	standard conditions	Prim-15	unspecified	ISH	Fig. S2
csf1ra	hdac1^b382/b382	standard conditions	Long-pec	xanthophore	ISH	Fig. 3

1 - 10 of 92

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO3-foxd3	standard conditions	Long-pec	sympathetic neuron absent, abnormal	Fig. S1
WT + MO3-foxd3	standard conditions	Protruding-mouth	melanophore stripe melanocyte amount, normal	Fig. 6
WT + MO3-foxd3	standard conditions	Protruding-mouth	melanophore stripe width, normal	Fig. 6
WT + MO3-foxd3	standard conditions	Protruding-mouth	yolk melanophore stripe present, normal	Fig. 6
WT + MO3-foxd3	standard conditions	Long-pec	sympathetic nervous system neuron absent, abnormal	Fig. S1
WT + MO3-foxd3	standard conditions	Long-pec	sympathetic nervous system neuron decreased amount, abnormal	Fig. S1
foxd3^zdf10/zdf10	standard conditions	Protruding-mouth	melanocyte decreased amount, abnormal	Fig. 7
foxd3^zdf10/zdf10	standard conditions	Protruding-mouth	melanocyte position, normal	Fig. 7
foxd3^zdf10/zdf10	standard conditions	Protruding-mouth	melanophore stripe melanocyte amount, normal	Fig. 7
foxd3^zdf10/zdf10	standard conditions	Protruding-mouth	melanophore stripe width, normal	Fig. 7

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b382		Point Mutation	hdac1
zdf10		Small Deletion	foxd3

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
foxd3	MO3-foxd3	MRPHLNO

Fish

Fish
foxd3^zdf10/zdf10
hdac1^b382/b382
hdac1^b382/b382; foxd3^zdf10/+
hdac1^b382/b382; foxd3^zdf10/+ + MO3-foxd3
hdac1^b382/b382; foxd3^zdf10/zdf10
hdac1^b382/b382; foxd3^zdf10/zdf10 + MO3-foxd3
hdac1^b382/b382 + MO3-foxd3
WT
WT + MO3-foxd3

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

Ignatius et al., 2008 ZDB-PUB-071219-5 PMID:18068699

colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis

Ignatius et al., 2008

ZDB-PUB-071219-5
PMID:18068699