ZFIN ID: ZDB-PUB-071210-25
Expression profiling identifies novel Hh/Gli-regulated genes in developing zebrafish embryos
Bergeron, S.A., Milla, L.A., Villegas, R., Shen, M.C., Burgess, S.M., Allende, M.L., Karlstrom, R.O., and Palma, V.
Date: 2008
Source: Genomics   91(2): 165-177 (Journal)
Registered Authors: Allende, Miguel L., Bergeron, Sadie, Burgess, Shawn, Karlstrom, Rolf, Milla, Luis
Keywords: Hedgehog, Detour (dtr), Slow muscle omitted (smu), Interrenal gland, Pronephros, Nervous system, Microarray, Transcriptional profiling
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Hedgehog Proteins/genetics*
  • Neoplasms/etiology
  • Neoplasms/genetics
  • Oncogene Proteins/genetics*
  • Signal Transduction
  • Tissue Distribution
  • Trans-Activators/genetics*
  • Zebrafish
PubMed: 18055165 Full text @ Genomics
The Hedgehog (Hh) signaling pathway plays critical instructional roles during embryonic development. Misregulation of Hh/Gli signaling is a major causative factor in human congenital disorders and in a variety of cancers. The zebrafish is a powerful genetic model for the study of Hh signaling during embryogenesis, as a large number of mutants that affect different components of the Hh/Gli signaling system have been identified. By performing global profiling of gene expression in different Hh/Gli gain- and loss-of-function scenarios we identified known (e.g., ptc1 and nkx2.2a) and novel Hh-regulated genes that are differentially expressed in embryos with altered Hh/Gli signaling function. By uncovering changes in tissue-specific gene expression, we revealed new embryological processes that are influenced by Hh signaling. We thus provide a comprehensive survey of Hh/Gli-regulated genes during embryogenesis and we identify new Hh-regulated genes that may be targets of misregulation during tumorigenesis.