ZFIN ID: ZDB-PUB-071129-1
Differential expression of duplicated VAL-opsin genes in the developing zebrafish
Kojima, D., Torii, M., Fukada, Y., and Dowling, J.E.
Date: 2008
Source: Journal of neurochemistry   104(5): 1364-1371 (Journal)
Registered Authors: Dowling, John E., Fukada, Yoshitaka, Kojima, Daisuke
Keywords: none
MeSH Terms:
  • Animals
  • Gene Expression Regulation, Developmental/physiology*
  • Genes, Duplicate/physiology*
  • MafB Transcription Factor/biosynthesis*
  • MafB Transcription Factor/genetics*
  • Nerve Tissue Proteins/biosynthesis*
  • Nerve Tissue Proteins/genetics*
  • Phylogeny
  • Protein Isoforms/biosynthesis
  • Protein Isoforms/genetics
  • Retinal Horizontal Cells/embryology
  • Retinal Horizontal Cells/physiology
  • Rod Opsins/biosynthesis*
  • Rod Opsins/genetics*
  • Zebrafish
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics*
PubMed: 18036148 Full text @ J. Neurochem.
Non-visual opsins mediate various light-dependent physiological events. Our previous search for non-visual opsin genes in zebrafish led to the discovery of VAL-opsin (VAL-opsinA) in deep brain cells and retinal horizontal cells of the adult fish. In this study, we report the identification and characterization of its duplicated gene, VAL-opsinB, in zebrafish. A molecular phylogenetic analysis indicates that VAL-opsinB is orthologous to a previously reported salmon gene and that the duplication of the VAL-opsin gene occurred in the teleost lineage. The recombinant protein of zebrafish VAL-opsinB forms a green-sensitive photopigment when reconstituted with 11-cis-retinal. VAL-opsinB expression was detected in a limited number of cells of the brain and the eye, and the expression pattern is distinct from that of the VAL-opsinA gene. Such a differential expression pattern suggests that VAL-opsinA and VAL-opsinB are involved in different physiological events in zebrafish.