PUBLICATION
Differential expression of duplicated VAL-opsin genes in the developing zebrafish
- Authors
- Kojima, D., Torii, M., Fukada, Y., and Dowling, J.E.
- ID
- ZDB-PUB-071129-1
- Date
- 2008
- Source
- Journal of neurochemistry 104(5): 1364-1371 (Journal)
- Registered Authors
- Dowling, John E., Fukada, Yoshitaka, Kojima, Daisuke
- Keywords
- none
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental/physiology*
- Genes, Duplicate/physiology*
- MafB Transcription Factor/biosynthesis*
- MafB Transcription Factor/genetics*
- Nerve Tissue Proteins/biosynthesis*
- Nerve Tissue Proteins/genetics*
- Phylogeny
- Protein Isoforms/biosynthesis
- Protein Isoforms/genetics
- Retinal Horizontal Cells/embryology
- Retinal Horizontal Cells/physiology
- Rod Opsins/biosynthesis*
- Rod Opsins/genetics*
- Zebrafish
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics*
- PubMed
- 18036148 Full text @ J. Neurochem.
Citation
Kojima, D., Torii, M., Fukada, Y., and Dowling, J.E. (2008) Differential expression of duplicated VAL-opsin genes in the developing zebrafish. Journal of neurochemistry. 104(5):1364-1371.
Abstract
Non-visual opsins mediate various light-dependent physiological events. Our previous search for non-visual opsin genes in zebrafish led to the discovery of VAL-opsin (VAL-opsinA) in deep brain cells and retinal horizontal cells of the adult fish. In this study, we report the identification and characterization of its duplicated gene, VAL-opsinB, in zebrafish. A molecular phylogenetic analysis indicates that VAL-opsinB is orthologous to a previously reported salmon gene and that the duplication of the VAL-opsin gene occurred in the teleost lineage. The recombinant protein of zebrafish VAL-opsinB forms a green-sensitive photopigment when reconstituted with 11-cis-retinal. VAL-opsinB expression was detected in a limited number of cells of the brain and the eye, and the expression pattern is distinct from that of the VAL-opsinA gene. Such a differential expression pattern suggests that VAL-opsinA and VAL-opsinB are involved in different physiological events in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping