PUBLICATION

Genetic analysis of the vertebrate hedgehog-signaling pathway using muscle cell fate specification in the zebrafish embryo

Authors
Roy, S.
ID
ZDB-PUB-071125-24
Date
2007
Source
Methods in molecular biology (Clifton, N.J.)   397(1): 55-66 (Chapter)
Registered Authors
Roy, Sudipto
Keywords
Hedgehog, zebrafish, slow muscle, muscle pioneer, fast muscle, antibody labeling
MeSH Terms
  • Animals
  • Antibodies
  • Body Patterning/genetics
  • Cell Lineage/genetics*
  • Chorion
  • Embryo, Nonmammalian/cytology*
  • Hedgehog Proteins/genetics*
  • Microscopy, Confocal
  • Molecular Biology/methods*
  • Muscle Cells/cytology*
  • Muscle Cells/metabolism
  • Signal Transduction/genetics*
  • Staining and Labeling
  • Tissue Fixation
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
18025713 Full text @ Meth. Mol. Biol.
Abstract
Over the recent years, a large number of embryological studies with the zebrafish have provided substantial evidence of its usefulness for the investigation of the genetic and cellular basis of vertebrate development. With regard to the Hedgehog (Hh) pathway, forward as well as reverse genetic approaches in this organism have not only validated the roles of evolutionarily conserved players of the signaling cascade, but have also contributed to the isolation of several novel components that had remained unidentified through screens in other animal models. Here, the author describes a whole mount antibody labeling method that allows the detection of three unique muscle cell fates in the zebrafish embryo, which are induced by distinct levels and timing of Hh-signaling activity. This technique provides a rapid and convenient assay that can be utilized for the evaluation of effects of loss- or gain-of-function of any gene on the levels of Hh pathway activation during embryogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping