ZFIN ID: ZDB-PUB-071125-24
Genetic analysis of the vertebrate hedgehog-signaling pathway using muscle cell fate specification in the zebrafish embryo
Roy, S.
Date: 2007
Source: Methods in molecular biology (Clifton, N.J.)   397(1): 55-66 (Chapter)
Registered Authors: Roy, Sudipto
Keywords: Hedgehog, zebrafish, slow muscle, muscle pioneer, fast muscle, antibody labeling
MeSH Terms:
  • Animals
  • Antibodies
  • Body Patterning/genetics
  • Cell Lineage/genetics*
  • Chorion
  • Embryo, Nonmammalian/cytology*
  • Hedgehog Proteins/genetics*
  • Microscopy, Confocal
  • Molecular Biology/methods*
  • Muscle Cells/cytology*
  • Muscle Cells/metabolism
  • Signal Transduction/genetics*
  • Staining and Labeling
  • Tissue Fixation
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 18025713 Full text @ Meth. Mol. Biol.
Over the recent years, a large number of embryological studies with the zebrafish have provided substantial evidence of its usefulness for the investigation of the genetic and cellular basis of vertebrate development. With regard to the Hedgehog (Hh) pathway, forward as well as reverse genetic approaches in this organism have not only validated the roles of evolutionarily conserved players of the signaling cascade, but have also contributed to the isolation of several novel components that had remained unidentified through screens in other animal models. Here, the author describes a whole mount antibody labeling method that allows the detection of three unique muscle cell fates in the zebrafish embryo, which are induced by distinct levels and timing of Hh-signaling activity. This technique provides a rapid and convenient assay that can be utilized for the evaluation of effects of loss- or gain-of-function of any gene on the levels of Hh pathway activation during embryogenesis.