PUBLICATION
Locomotor pattern in the adult zebrafish spinal cord in vitro
- Authors
- Gabriel, J.P., Mahmood, R., Walter, A.M., Kyriakatos, A., Hauptmann, G., Calabrese, R.L., and El Manira, A.
- ID
- ZDB-PUB-071118-21
- Date
- 2008
- Source
- Journal of neurophysiology 99(1): 37-48 (Journal)
- Registered Authors
- Hauptmann, Giselbert
- Keywords
- none
- MeSH Terms
-
- Action Potentials/drug effects
- Action Potentials/physiology
- Animals
- Biological Clocks/drug effects
- Biological Clocks/physiology
- Excitatory Amino Acid Agonists/pharmacology
- Functional Laterality/physiology
- Glycine/antagonists & inhibitors
- Glycine/metabolism
- Locomotion/drug effects
- Locomotion/physiology*
- Models, Biological
- Motor Neurons/cytology
- Motor Neurons/drug effects
- Motor Neurons/physiology*
- Nerve Net/cytology
- Nerve Net/drug effects
- Nerve Net/physiology*
- Neural Pathways/physiology
- Patch-Clamp Techniques
- Periodicity
- Spinal Cord/cytology
- Spinal Cord/physiology*
- Spinal Nerve Roots/drug effects
- Spinal Nerve Roots/physiology
- Strychnine/pharmacology
- Synaptic Transmission/drug effects
- Synaptic Transmission/physiology
- Zebrafish/anatomy & histology
- Zebrafish/physiology*
- PubMed
- 17977928 Full text @ J. Neurophysiol.
Citation
Gabriel, J.P., Mahmood, R., Walter, A.M., Kyriakatos, A., Hauptmann, G., Calabrese, R.L., and El Manira, A. (2008) Locomotor pattern in the adult zebrafish spinal cord in vitro. Journal of neurophysiology. 99(1):37-48.
Abstract
The zebrafish is an attractive model system for studying the function of the spinal locomotor network by combining electrophysiological, imaging and genetic approaches. Thus far, most studies have been focusing on embryonic and larval stages. In this study we have developed an in vitro preparation of the isolated spinal cord from adult zebrafish in which locomotor activity can be induced while the activity of single neurons can be monitored using whole-cell recording techniques. Application of NMDA elicited rhythmic locomotor activity that was monitored by recording from muscles or ventral roots in semi-intact or isolated spinal cord preparations, respectively. This rhythmic activity displayed a left-right alternation and a rostro-caudal delay. Blockade of glycinergic synaptic transmission by strychnine switched the alternating activity into synchronous bursting in the left and right sides as well as along the rostro-caudal axis. Whole-cell recordings from motoneurons showed that they receive phasic synaptic inputs that were correlated with the locomotor activity recorded in ventral roots. This newly developed in vitro preparation of the adult zebrafish spinal cord will allow the examination of the organization of the spinal locomotor network in an adult system to complement studies in zebrafish larvae and new born rodents.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping