PUBLICATION

The zebrafish, brain-specific, aromatase cyp19a2 is neither expressed nor distributed in a sexually dimorphic manner during sexual differentiation

Authors
Kallivretaki, E., Eggen, R.I., Neuhauss, S.C., Kah, O., and Segner, H.
ID
ZDB-PUB-071023-6
Date
2007
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   236(11): 3155-3166 (Journal)
Registered Authors
Kah, Olivier, Neuhauss, Stephan
Keywords
aromatase, zebrafish, sexual differentiation, sexual dimorphism, brain, gonad
MeSH Terms
  • Animals
  • Aromatase/genetics*
  • Aromatase/metabolism*
  • Brain/cytology
  • Brain/embryology
  • Brain/enzymology*
  • Brain/growth & development
  • Female
  • Gene Expression Regulation, Developmental
  • Male
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sex Characteristics
  • Sex Differentiation*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed
17937394 Full text @ Dev. Dyn.
Abstract
Differential cyp19 aromatase expression during development leads to sexual dimorphisms in the mammalian brain. Whether this is also true for fish is unknown. The aim of the current study has been to follow the expression of the brain-specific aromatase cyp19a2 in the brains of sexually differentiating zebrafish. To assess the role of cyp19a2 in the zebrafish brain during gonadal differentiation, we used quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry to detect differences in the transcript or protein levels and/or expression pattern in juvenile fish, histology to monitor the gonadal status, and double immunofluorescence with neuronal or radial glial markers to characterize aromatase-positive cells. Our data show that cyp19a2 expression levels during zebrafish sexual differentiation cannot be assigned to a particular sex; the expression pattern in the brain is similar in both sexes and aromatase-positive cells appear to be mostly of radial glial nature.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping