PUBLICATION
Mannose binding lectin (MBL) copy number polymorphism in Zebrafish (D. rerio) and identification of haplotypes resistant to L. anguillarum
- Authors
- Jackson, A.N., McLure, C.A., Dawkins, R.L., and Keating, P.J.
- ID
- ZDB-PUB-071023-11
- Date
- 2007
- Source
- Immunogenetics 59(11): 861-872 (Journal)
- Registered Authors
- Keywords
- Zebrafish, Lectin pathway, MBL, Ancestral haplotypes, Genomic matching technique, L. anguillarum
- MeSH Terms
-
- Animals
- Base Sequence
- Crosses, Genetic
- DNA Primers/genetics
- DNA, Bacterial/genetics
- Female
- Fish Diseases/genetics
- Fish Diseases/immunology
- Fish Diseases/microbiology
- Fisheries
- Gene Dosage*
- Gram-Negative Bacterial Infections/genetics
- Gram-Negative Bacterial Infections/immunology
- Gram-Negative Bacterial Infections/veterinary
- Haplotypes
- Immunity, Innate
- Listonella/pathogenicity*
- Male
- Mannose-Binding Lectin/genetics*
- Mannose-Binding Lectin/immunology*
- Molecular Sequence Data
- Multigene Family
- Phylogeny
- Polymorphism, Genetic
- Sequence Homology, Nucleic Acid
- Zebrafish/genetics*
- Zebrafish/immunology*
- Zebrafish/microbiology
- PubMed
- 17943278 Full text @ Immunogenetics
Citation
Jackson, A.N., McLure, C.A., Dawkins, R.L., and Keating, P.J. (2007) Mannose binding lectin (MBL) copy number polymorphism in Zebrafish (D. rerio) and identification of haplotypes resistant to L. anguillarum. Immunogenetics. 59(11):861-872.
Abstract
We describe a novel extension of the Genomic Matching Technique (GMT) that defines haplotypes of the mannose binding lectin (MBL) region in Zebrafish (D. rerio). Four ancestral haplotypes have been identified to date, with at least one of these demonstrating a significant increase in resistance to L. anguillarum. MBL activates the lectin pathway of the complement system and stimulates the development of the complement cascade and the Membrane Attack Complex. Polymorphisms in humans have been associated with increased susceptibility and severity to a number of pathogenic organisms. As teleosts have a relatively immature acquired immune system, polymorphisms within MBL and other innate defence genes are likely to be critical in defining their susceptibility/resistance to various pathogenic organisms. We report multiple copies of MBL-like genes in D. rerio, with up to three copies tightly linked within a cluster spanning approximately 15 kb on chromosome 2. Genomic analysis suggests that duplication, retroviral insertion and possibly gene mutation and/or deletion have been key factors in the evolution of this cluster. Molecular analysis has revealed extensive polymorphism, including at least five distinct amplicons and haplospecific gene copy number variation. This study demonstrates polymorphism within a critical component of the teleost innate immune system. The polymorphisms and the haplotypes encoding the unique variants are likely to be informative in defining susceptibility/resistance to infectious agents commonly encountered within aquatic environments. Future investigations will define other important haplotypes and transfer the knowledge to other finfish species, thereby enabling selection of broodstock for the aquaculture industry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping