PUBLICATION
Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron
- Authors
- Hardy, M.E., Ross, L.V., and Chien, C.B.
- ID
- ZDB-PUB-071009-4
- Date
- 2007
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 236(11): 3071-3076 (Journal)
- Registered Authors
- Chien, Chi-Bin, Hardy, Melissa, Ross, Louis
- Keywords
- transgene, conditional expression, hsp70, GFP
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/metabolism*
- Gene Expression Regulation, Developmental*
- HSP70 Heat-Shock Proteins/genetics
- Heat-Shock Response*
- Hot Temperature*
- Intercellular Signaling Peptides and Proteins/genetics
- Nerve Tissue Proteins/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 17907198 Full text @ Dev. Dyn.
Citation
Hardy, M.E., Ross, L.V., and Chien, C.B. (2007) Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(11):3071-3076.
Abstract
Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue-specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utility. Here we describe a new method of misexpression: local heat shock using a modified soldering iron. Zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) are focally heated with the soldering iron to induce gene expression in a small area of the embryo. We have validated this method in three stable transgenic lines and at three developmental timepoints. Local heat shock is a fast, easy, and inexpensive method for gene misexpression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping