ZFIN ID: ZDB-PUB-071009-4
Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron
Hardy, M.E., Ross, L.V., and Chien, C.B.
Date: 2007
Source: Developmental dynamics : an official publication of the American Association of Anatomists   236(11): 3071-3076 (Journal)
Registered Authors: Chien, Chi-Bin, Hardy, Melissa, Ross, Louis
Keywords: transgene, conditional expression, hsp70, GFP
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression Regulation, Developmental*
  • HSP70 Heat-Shock Proteins/genetics
  • Heat-Shock Response*
  • Hot Temperature*
  • Intercellular Signaling Peptides and Proteins/genetics
  • Nerve Tissue Proteins/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 17907198 Full text @ Dev. Dyn.
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ABSTRACT
Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue-specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utility. Here we describe a new method of misexpression: local heat shock using a modified soldering iron. Zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) are focally heated with the soldering iron to induce gene expression in a small area of the embryo. We have validated this method in three stable transgenic lines and at three developmental timepoints. Local heat shock is a fast, easy, and inexpensive method for gene misexpression.
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