ZFIN ID: ZDB-PUB-071009-16
Vestigial-like-2b (VITO-1b) and Tead-3a (Tef-5a) expression in zebrafish skeletal muscle, brain and notochord
Mann, C.J., Osborn, D.P., and Hughes, S.M.
Date: 2007
Source: Gene expression patterns : GEP   7(8): 827-836 (Journal)
Registered Authors: Hughes, Simon M., Osborn, Dan
Keywords: Muscle, Adaxial, Zebrafish, Vestigial-like, Transcription enhancer factor, TEAD domain, Vgl, Skeletal muscle, VITO, Vestigial, Notochord, TEAD, Tef, Scalloped, Rhombomere, Eye; Epiphysis, Somite, Gene expression, Floatinghead, Cyclopamine, Smoothened, After eight, Fused somites, Danio rerio
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Brain/embryology
  • Brain/metabolism
  • Central Nervous System/chemistry
  • Central Nervous System/embryology
  • Cloning, Molecular
  • Gene Expression
  • Molecular Sequence Data
  • Muscle, Skeletal/chemistry
  • Muscle, Skeletal/embryology
  • Muscle, Skeletal/metabolism
  • Notochord/chemistry
  • Notochord/embryology
  • Notochord/metabolism
  • Phylogeny
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Transcription Factors/chemistry
  • Transcription Factors/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
PubMed: 17916448 Full text @ Gene Expr. Patterns
The vestigial gene has been shown to control skeletal muscle formation in Drosophila and the related Vestigial-like 2 (Vgl-2) protein plays a similar role in mice. Vgl-family proteins are thought to regulate tissue-specific gene expression by binding to members of the broadly expressed Scalloped/Tef/TEAD transcription factor family. Zebrafish have at least four Vgl genes, including two Vgl-2s, and at least three TEAD genes, including two Tead3s. We describe the cloning and expression of one member from each family in the zebrafish. A novel gene, vgl-2b, with closest homology to mouse and human vgl-2, is expressed transiently in nascent notochord and in muscle fibres as they undergo terminal differentiation during somitogenesis. Muscle cells also express a TEAD-3 homologue, a possible partner of Vgl-2b, during myoblast differentiation and early fibre assembly. Tead-3a is also expressed in rhombomeres, eye and epiphysis regions.