PUBLICATION
Genome-wide analysis and expression profiling of the small heat shock proteins in zebrafish
- Authors
- Elicker, K.S., and Hutson, L.D.
- ID
- ZDB-PUB-071001-2
- Date
- 2007
- Source
- Gene 403(1-2): 60-69 (Journal)
- Registered Authors
- Hutson, Lara
- Keywords
- sHSP, α-Crystallin, Synteny, Real time PCR, Development
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Conserved Sequence
- Databases, Genetic
- Embryo, Nonmammalian
- Evolution, Molecular
- Expressed Sequence Tags
- Gene Expression Profiling/methods*
- Gene Expression Regulation, Developmental*
- Genome*
- Heat-Shock Proteins/genetics*
- Molecular Sequence Data
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, Protein
- Sequence Homology, Amino Acid
- Synteny
- Zebrafish/embryology
- Zebrafish/genetics*
- alpha-Crystallins/genetics
- PubMed
- 17888590 Full text @ Gene
Citation
Elicker, K.S., and Hutson, L.D. (2007) Genome-wide analysis and expression profiling of the small heat shock proteins in zebrafish. Gene. 403(1-2):60-69.
Abstract
Small Heat Shock Proteins (sHSPs) have important roles in preventing disease and promoting resistance to environmental stressors. Mutations in any one of a number of sHSPs, including HSP27 (HSPB1), HSP22 (HSPB8), alphaA-crystallin (HSPB4), or alphaB-crystallin (HSPB5) can result in neuronal degeneration, myopathy, and/or cataract in humans. Ten sHSPs are known in humans, and thirteen have been identified in teleost fish. Here we report the identification of thirteen zebrafish sHSPs. Using a combination of phylogenetic analysis and analysis of synteny, we have determined that ten are likely orthologs of human sHSPs. We have used quantitative RT-PCR to determine the relative expression levels of all thirteen sHSPs during development and in response to heat shock. Our findings indicate that most of the zebrafish sHSPs are expressed during development, and five of these genes are transcriptionally upregulated by heat shock at one or more stages of development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping