PUBLICATION

Convergence and extension movements affect dynamic notochord-somite interactions essential for zebrafish slow muscle morphogenesis

Authors

Yin, C., and Solnica-Krezel, L.

ID

ZDB-PUB-070920-4

Date

2007

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 236(10): 2742-2756 (Journal)

Registered Authors

Solnica-Krezel, Lilianna, Yin, Chunyue

Keywords

adaxial cell, knypek, trilobite, non-canonical Wnt, fibronectin, somitic boundary, gastrulation

MeSH Terms

Gene Expression Regulation, Developmental
Muscle Development*
Animals
Hedgehog Proteins/metabolism
Somites/physiology*
Muscle Cells/cytology
Muscle Cells/physiology*
Embryonic Development
Cell Movement
Notochord/physiology*
Zebrafish/embryology*
Zebrafish/genetics
Body Patterning
Mutation
Zebrafish Proteins/metabolism

(all 15)

PubMed

17849437 Full text @ Dev. Dyn.

Abstract

During vertebrate gastrulation, convergence and extension (C&E) movements shape and position the somites that form the fast and slow muscles. In zebrafish knypek;trilobite non-canonical Wnt mutants, defective C&E movements cause misshapen somites and reduction of slow muscle precursors, the adaxial cells. Here, we demonstrate essential roles of C&E in slow muscle morphogenesis. During segmentation, the adaxial cells change shapes and migrate laterally to form slow muscles at the myotome surface. Using confocal imaging techniques, we show that the adaxial cells undergo three-step shape changes, including dorsoventral elongation, anterior-ward rotation, and anteroposterior elongation. The adaxial cells in knypek;trilobite double mutants maintain prolonged contact with the notochord and fail to rotate anteriorly. Such a defect was suppressed by physical removal of their notochord or by introducing wild-type notochord cells into the mutant. We propose that in the double mutants, impaired C&E movements disrupt notochord development, which impedes the adaxial cell shape changes.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
gpc4^m119/m119	standard conditions	5-9 somites	adaxial cell shape, abnormal	Fig. 2
gpc4^m119/m119	standard conditions	5-9 somites	cell morphogenesis disrupted, abnormal	Fig. 2
gpc4^m119/m119	standard conditions	14-19 somites to Long-pec	muscle cell migration disrupted, abnormal	Fig. 1
gpc4^m119/m119	standard conditions	14-19 somites to Long-pec	slow muscle cell decreased length, abnormal	Fig. 1
gpc4^m119/m119	standard conditions	14-19 somites to Long-pec	slow muscle cell disorganized, abnormal	Fig. 1
gpc4^m119/m119	standard conditions	14-19 somites to Long-pec	striated muscle cell development disrupted, abnormal	Fig. 1
gpc4^m119/m119; vangl2^m209/m209	standard conditions	5-9 somites	adaxial cell shape, abnormal	Fig. 2
gpc4^m119/m119; vangl2^m209/m209	standard conditions	5-9 somites	cell morphogenesis disrupted, abnormal	Fig. 2
gpc4^m119/m119; vangl2^m209/m209	standard conditions	10-13 somites	notochord cell circular, abnormal	Fig. 7
gpc4^m119/m119; vangl2^m209/m209	standard conditions	10-13 somites	notochord increased width, abnormal	Fig. 7

1 - 10 of 24

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
m119	Point Mutation	gpc4
m209	Point Mutation	vangl2
n1	Small Deletion	noto
tbx6_unspecified	Unspecified	tbx6

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
gpc4^m119/m119
gpc4^m119/m119; vangl2^m209/m209
gpc4^m119/m119; vangl2^m209/m209; tbx6^unspecified
noto^n1/n1
tbx6^unspecified
vangl2^m209/m209
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Yin et al., 2007 ZDB-PUB-070920-4 PMID:17849437

Convergence and extension movements affect dynamic notochord-somite interactions essential for zebrafish slow muscle morphogenesis

Yin et al., 2007

ZDB-PUB-070920-4
PMID:17849437