PUBLICATION
Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation
- Authors
- Akanuma, T., Koshida, S., Kawamura, A., Kishimoto, Y., and Takada, S.
- ID
- ZDB-PUB-070907-8
- Date
- 2007
- Source
- EMBO reports 8(9): 858-863 (Journal)
- Registered Authors
- Akanuma, Takashi, Kawamura, Akinori, Kishimoto, Yasuyuki, Koshida, Sumito, Takada, Shinji
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning/genetics*
- Gene Expression Regulation, Developmental
- Mutation/genetics
- Nuclear Proteins/chemistry*
- Receptors, Notch/metabolism*
- Saccharomyces cerevisiae Proteins/chemistry*
- Sequence Homology, Amino Acid
- Signal Transduction
- Somites/cytology
- Somites/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Transcription, Genetic*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 17721442 Full text @ EMBO Rep.
Citation
Akanuma, T., Koshida, S., Kawamura, A., Kishimoto, Y., and Takada, S. (2007) Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation. EMBO reports. 8(9):858-863.
Abstract
Members of the yeast polymerase-associated factor 1 (Paf1) complex, which is composed of at least five components (Paf1, Rtf1, Cdc73, Leo1 and Ctr9), are conserved from yeast to humans. Although these proteins have been implicated in RNA polymerase II-mediated transcription, their roles in vertebrate development have not been explained. Here, we show that a zebrafish mutant with a somite segmentation defect is deficient in rtf1. In addition, embryos deficient in rtf1 or ctr9 show abnormal development of the heart, ears and neural crest cells. rtf1 is required for correct RNA levels of the Notch-regulated genes her1, her7 and deltaC, and also for Notch-induced her1 expression in the presomitic mesoderm. Furthermore, the phenotype observed in rtf1-deficient mutants is enhanced by an additional deficiency in mind bomb, which encodes an effector of Notch signalling. Therefore, zebrafish homologues of the yeast Paf1 complex seem to preferentially affect a subset of genes, including Notch-regulated genes, during embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping