PUBLICATION

Light-controlled gene silencing in zebrafish embryos

Authors
Shestopalov, I.A., Sinha, S., and Chen, J.K.
ID
ZDB-PUB-070827-28
Date
2007
Source
Nature Chemical Biology   3(10): 650-651 (Journal)
Registered Authors
Chen, James K., Sinha, Surajit
Keywords
none
MeSH Terms
  • Glycoproteins/biosynthesis
  • Glycoproteins/chemistry
  • Glycoproteins/genetics
  • Body Patterning
  • Embryo, Nonmammalian/physiology
  • Embryo, Nonmammalian/radiation effects*
  • Gene Silencing/physiology
  • Gene Silencing/radiation effects*
  • Animals
  • RNA Splicing/drug effects*
  • RNA Splicing/physiology
  • Ultraviolet Rays
  • Light*
  • Peptide Chain Initiation, Translational
  • Gene Expression Regulation, Developmental/physiology
  • Gene Expression Regulation, Developmental/radiation effects*
  • Zebrafish
(all 17)
PubMed
17717538 Full text @ Nat. Chem. Biol.
Abstract
Functional genomic studies in zebrafish frequently use synthetic oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tc41
    Unknown
    1 - 1 of 1
    Show
    Human Disease / Model
    Sequence Targeting Reagents
    Fish
    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping