PUBLICATION

Expression pattern for unc5b, an axon guidance gene in embryonic zebrafish development

Authors
Kaur, S., Abu-Abab, M.S., Singla, S., Yeo, S.Y., and Ramchandran, R.
ID
ZDB-PUB-070827-10
Date
2007
Source
Gene Expression   13(6): 321-327 (Journal)
Registered Authors
Ramchandran, Ramani, Yeo, Sang-Yeob
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Axons/physiology*
  • Body Patterning/genetics
  • Cloning, Molecular
  • Embryo, Nonmammalian
  • Embryonic Development/genetics*
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Phylogeny
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • Receptors, Cell Surface/physiology*
  • Rhombencephalon/embryology
  • Sequence Homology, Amino Acid
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed
17708418 Full text @ Gene Expr.
Abstract
Branching processes such as nerves and vessels share molecular mechanisms of path determination. Our study focuses on unc5b, a member of the unc5 axon guidance gene family. Here, we have cloned the full-length zebrafish ortholog of unc5b, mapped its chromosome location in the zebrafish genome, and compared its expression patterns to robo4, another axon guidance family member. In situ show that unc5b is expressed predominantly in sensory structures such as the eye, ear, and brain. Both unc5b and robo4 show robust expression in all three compartments of the embryonic brain, namely forebrain, midbrain, and hindbrain. In particular, the hindbrain rhombomere expression displays interesting patterns in that robo4 is expressed in medial rhombomere cell clusters when compared to unc5b expressed in lateral rhombomere clusters. A similar medial-lateral theme is observed in other neural structures such as the neural tube. Our expression analysis provides a starting point for studying the role of axon guidance genes in embryonic hindbrain patterning.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping