PUBLICATION
Molecular changes associated with teratogen-induced cyclopia
- Authors
- Loucks, E.J., Schwend, T., and Ahlgren, S.C.
- ID
- ZDB-PUB-070806-9
- Date
- 2007
- Source
- Birth defects research. Part A, Clinical and molecular teratology 79(9): 642-651 (Journal)
- Registered Authors
- Ahlgren, Sara, Loucks, Evyn
- Keywords
- zebrafish, cyclopia, gli, six3b, dlx3b, telencephalon
- MeSH Terms
-
- Animals
- Base Sequence
- Colforsin/toxicity
- DNA Primers/genetics
- Ethanol/toxicity
- Eye Abnormalities/chemically induced*
- Eye Abnormalities/embryology
- Eye Abnormalities/genetics*
- Eye Proteins/genetics
- Gene Expression Regulation, Developmental/drug effects
- Genes, Homeobox/drug effects
- Goosecoid Protein/genetics
- Hedgehog Proteins/genetics
- Homeodomain Proteins/genetics
- Kruppel-Like Transcription Factors/genetics
- Nerve Tissue Proteins/genetics
- Teratogens/toxicity*
- Veratrum Alkaloids/toxicity
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 17647295 Full text @ Birth Defects Res. Part A Clin. Mol. Teratol.
Citation
Loucks, E.J., Schwend, T., and Ahlgren, S.C. (2007) Molecular changes associated with teratogen-induced cyclopia. Birth defects research. Part A, Clinical and molecular teratology. 79(9):642-651.
Abstract
BACKGROUND: Exposure of zebrafish embryos to a number of teratogens results in cyclopia, but little is known about the underlying molecular changes. METHODS: Using zebrafish embryos, we compare the effects cyclopamine, forskolin, and ethanol delivered starting just before gastrulation, on gene expression in early axial tissues and forebrain development. RESULTS: Although all three teratogens suppress gli1 expression, they do so with variable kinetics, suggesting that while suppression of Shh signaling is a common outcome of these three teratogens, it is not a common cause of the cyclopia. Instead, all teratogens studied produce a series of changes in the expression of gsc and six3b present in early axial development, as well as a later suppression of neural crest cell marker dlx3b. Ethanol and forskolin, but not cyclopamine, exposure reduced anterior markers, which most likely contributes to the cyclopic phenotype. CONCLUSIONS: These data suggest that each teratogen exposure leads to a unique set of molecular changes that underlie the single phenotype of cyclopia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping