PUBLICATION

Zebrafish Hoxb1a regulates multiple downstream genes including prickle1b

Authors
Rohrschneider, M.R., Elsen, G.E., and Prince, V.E.
ID
ZDB-PUB-070806-13
Date
2007
Source
Developmental Biology   309(2): 358-372 (Journal)
Registered Authors
Elsen, Gina, Prince, Victoria E., Rohrschneider, Monica
Keywords
Zebrafish, Hox, Hindbrain, Rhombomere 4, Branchiomotor neurons, Facial neurons, Neuronal migration, Prickle
Datasets
GEO:GSE5199
MeSH Terms
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Carrier Proteins/biosynthesis*
  • Cell Movement
  • Expressed Sequence Tags
  • Face/innervation
  • Gene Expression Regulation
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • LIM Domain Proteins
  • Motor Neurons/physiology
  • Oligonucleotide Array Sequence Analysis
  • Signal Transduction
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/biosynthesis*
PubMed
17651720 Full text @ Dev. Biol.
Abstract
Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene plays a critical and conserved role in vertebrate hindbrain development, conferring identity to hindbrain rhombomere (r) 4. For example, zebrafish Hoxb1a, similar to mouse Hoxb1, is required for the migration of r4-derived facial branchiomotor neurons into the posterior hindbrain. We have screened microarrays carrying more than 16,000 expressed sequence tags (ESTs) for genes that are differentially regulated in normal versus Hoxb1a-deficient r4 tissue. Using this approach, we have identified both positively and negatively regulated candidate Hoxb1a target genes. We have used in situ hybridization to validate twelve positively regulated Hoxb1a targets. These downstream targets are expressed in a variety of subdomains within r4, with one gene, a novel prickle homolog (pk1b), expressed specifically within the facial branchiomotor neurons. Using morpholino knock-down and cell transplantation, we demonstrate that the Hoxb1a target Prickle1b functions cell-autonomously to control facial neuron migration, a single aspect of r4 identity.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes