Functional conservation of erythropoietin signaling in zebrafish
- Paffett-Lugassy, N., Hsia, N., Fraenkel, P.G., Paw, B., Leschinsky, I., Barut, B., Bahary, N., Caro, J., Handin, R., and Zon, L.I.
- Blood 110(7): 2718-2726 (Journal)
- Registered Authors
- Bahary, Nathan, Barut, Bruce, Fraenkel, Paula, Handin, Robert, Hsia, Nelson, Paffett-Lugassy, Noelle, Paw, Barry, Zon, Leonard I.
- MeSH Terms
- Amino Acid Sequence
- Conserved Sequence
- DNA, Complementary/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Erythroid Cells/cytology
- Erythropoietin/isolation & purification
- Gene Expression Regulation, Developmental
- Molecular Sequence Data
- Receptors, Erythropoietin/chemistry
- Receptors, Erythropoietin/genetics
- Receptors, Erythropoietin/metabolism
- STAT5 Transcription Factor/metabolism
- Sequence Alignment
- Signal Transduction*
- 17579187 Full text @ Blood
Paffett-Lugassy, N., Hsia, N., Fraenkel, P.G., Paw, B., Leschinsky, I., Barut, B., Bahary, N., Caro, J., Handin, R., and Zon, L.I. (2007) Functional conservation of erythropoietin signaling in zebrafish. Blood. 110(7):2718-2726.
Erythropoietin (Epo) and its cognate receptor (EpoR) are required for maintaining adequate levels of circulating erythrocytes during embryogenesis and adulthood. Here, we report the functional characterization of the zebrafish epo and epor genes. The expression of epo and epor was evaluated by quantitative RT-PCR and whole mount in situ hybridization, revealing marked parallels between zebrafish and mammalian gene expression patterns. Examination of the hypochromic mutant, weissherbst, and adult hypoxia-treated hearts indicate that zebrafish epo expression is induced by anemia and hypoxia. Overexpression of epo mRNA resulted in severe polycythemia, characterized by a striking increase in the number of cells expressing scl, c-myb, gata1, ikaros, epor, and betae1-globin, suggesting that both the erythroid progenitor and mature erythrocyte compartments respond to epo. Morpholino-mediated knockdown of the epor caused a slight decrease in primitive and complete block of definitive erythropoiesis. Abrogation of STAT5 blocked the erythropoietic expansion by epo mRNA, consistent with a requirement for STAT5 in epo signaling. Together, the characterization of zebrafish epo and epor demonstrates the conservation of an ancient program that ensures proper red blood cell numbers during normal homeostasis and under hypoxic conditions.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes