PUBLICATION

Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio

Authors
Yang, H., Carmichael, C., Varga, Z.M., and Tiersch, T.R.
ID
ZDB-PUB-070625-6
Date
2007
Source
Theriogenology   68(2): 128-136 (Journal)
Registered Authors
Carmichael, Carrie, Varga, Zoltan M.
Keywords
Sperm cryopreservation, In vitro fertilization, Zebrafish, Danio rerio
MeSH Terms
  • Animals
  • Cryopreservation/methods*
  • Cryopreservation/standards
  • Cryoprotective Agents/toxicity
  • Male
  • Methanol/toxicity
  • Osmolar Concentration
  • Sperm Motility/drug effects
  • Spermatozoa*/drug effects
  • Temperature
  • Zebrafish/physiology*
PubMed
17544099 Full text @ Theriogenology
Abstract
Sperm cryopreservation offers potential for long-term storage of genetic resources. However, the current protocols for zebrafish Danio rerio are cumbersome and poorly reproducible. Our objective was to facilitate adoption of cryopreservation by streamlining methods from sperm collection through thawing and use. First, sperm activation was evaluated, and motility was completely inhibited when osmolality of the extender was >/=295-300mOsmol/kg. To evaluate cryoprotectant toxicity, sperm were incubated with dimethyl sulfoxide (DMSO), N,N-dimethyl acetamide (DMA), methanol, or glycerol at 5, 10, and 15% concentrations. Based on motility, DMSO, DMA, and methanol (
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes