PUBLICATION
CNBP mediates neural crest cell expansion by controlling cell proliferation and cell survival during rostral head development
- Authors
- Weiner, A.M., Allende, M.L., Becker, T.S., and Calcaterra, N.B.
- ID
- ZDB-PUB-070513-1
- Date
- 2007
- Source
- Journal of cellular biochemistry 102(6): 1553-1570 (Journal)
- Registered Authors
- Allende, Miguel L., Becker, Thomas S., Calcaterra, Nora
- Keywords
- CNBP, Zebrafish, cell proliferation, cell survival, head development, craniofacial skeleton
- MeSH Terms
-
- Animals
- Cell Death/physiology
- Cell Proliferation*
- Cell Survival/physiology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/physiology
- Female
- Gene Expression Regulation, Developmental
- Head/embryology*
- In Situ Hybridization
- Microinjections
- Neural Crest/cytology
- Neural Crest/physiology*
- Oligonucleotides, Antisense/pharmacology
- RNA, Messenger/metabolism
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 17471504 Full text @ J. Cell. Biochem.
Citation
Weiner, A.M., Allende, M.L., Becker, T.S., and Calcaterra, N.B. (2007) CNBP mediates neural crest cell expansion by controlling cell proliferation and cell survival during rostral head development. Journal of cellular biochemistry. 102(6):1553-1570.
Abstract
Striking conservation in various organisms suggests that cellular nucleic acid binding protein (CNBP) plays a fundamental biological role across different species. Recently, it was reported that CNBP is required for forebrain formation during chick and mouse embryogenesis. In this study, we have used the zebrafish model system to expand and contextualize the basic understanding of the molecular mechanisms of CNBP activity during vertebrate head development. We show that zebrafish cnbp is expressed in the anterior CNS in a similar fashion as has been observed in early chick and mouse embryos. Using antisense morpholino oligonucleotide knockdown assays, we show that CNBP depletion causes forebrain truncation while trunk development appears normal. A substantial reduction in cell proliferation and an increase in cell death were observed in the anterior regions of cnbp morphant embryos, mainly within the cnbp expression territory. In situ hybridization assays show that CNBP depletion does not affect CNS patterning while it does cause depletion of neural crest derivatives. Our data suggest an essential role for CNBP in mediating neural crest expansion by controlling proliferation and cell survival rather than via a cell fate switch during rostral head development. This possible role of CNBP may not only explain the craniofacial anomalies observed in zebrafish but also those reported for mice and chicken and, moreover, demonstrates that CNBP plays an essential and conserved role during vertebrate head development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping