PUBLICATION
Knocked down and out: PACAP in development, reproduction and feeding
- Authors
- Sherwood, N.M., Adams, B.A., Isaac, E.R., Wu, S., and Fradinger, E.A.
- ID
- ZDB-PUB-070504-23
- Date
- 2007
- Source
- Peptides 28(9): 1680-1687 (Review)
- Registered Authors
- Fradinger, Erica, Sherwood, Nancy M., Wu, Shan-Fu
- Keywords
- Morpholino, PACAP gene knockout, Brain development, Reproduction, Energy balance, High fat diet, Mice, Zebrafish
- MeSH Terms
-
- Animals
- Brain/embryology*
- Brain/metabolism
- Eating/physiology
- Feeding Behavior/physiology
- Gene Deletion*
- Gene Expression Regulation, Developmental
- Mice
- Pituitary Adenylate Cyclase-Activating Polypeptide/genetics
- Pituitary Adenylate Cyclase-Activating Polypeptide/metabolism
- Pituitary Adenylate Cyclase-Activating Polypeptide/physiology*
- Reproduction/physiology
- Zebrafish
- PubMed
- 17467121 Full text @ Peptides
Citation
Sherwood, N.M., Adams, B.A., Isaac, E.R., Wu, S., and Fradinger, E.A. (2007) Knocked down and out: PACAP in development, reproduction and feeding. Peptides. 28(9):1680-1687.
Abstract
One approach to understanding the role of PACAP in vivo is to knockdown the translation of PACAP mRNA to protein or to knock out the PACAP gene by targeted disruption. In this paper, we review the effect of PACAP knockdown with morpholinos on early brain development in zebrafish. Also reviewed is the role of PACAP at several stages of reproduction as assessed in mice with a disrupted PACAP gene. New data are presented to analyze PACAP's action in energy homeostasis (body mass, food intake, endocrine parameters) using female PACAP-null mice. The evidence suggests PACAP is important for brain development in zebrafish and is required for normal reproduction, but not for body mass or food intake in mice maintained near thermoneutrality.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping